A Polymorphism Of Manganese Superoxide Dismutase Gene V (16) And Diabetic Nephropathy Related Research

The factors influencing polymerase chain reaction-restriction fragment length polymorphism in the site of MnSOD gene V(16)A polymorphism were studied. The results revealed the optimum conditions: denaturation temperature was 95℃; annealing temperature was 55℃; primer concentration was 0.25μmol/L; magnesium concentration was 1.5 mmol/L; concentration of dNTP was 0.20mmol/L ; Taq enzyme was 0.6 U; the effective digestion system was 15μ1 including 10μl DNA solution and 2.5U enzyme. The information in this study may be useful to relative experiments.