The Yin And Yang Pairs Make Up The Party And The Demolition Of The Side Protective Effect Of Oxidative Damage Of Pc12 Cells And Plc-gamma <sub> 1 </ Sub>-of Pkc¦Á In The Pld Signal Transduction Pathway Impact Study

Objective: The effects of enhancing Yang and nourishing Yin formula(YY) and its every separated formulas (HY, HG, YG, G, Y, H) on PLC-γ₁—PKCα—PLD signal transduction pathway in PC12 cells injured by H₂O₂ were observed to study the therapeutic mechanism of enhancing Yang and nourishing Yin formula and to discuss the relationship between Yang deficiency, Yin deficiency and free radical injury in neurological diseases.Methods: Establishing appropriate H₂O₂- injuring PC12 cell model with MTT assay. Screening the best formula by orthogonal tests. After PC12 cells were given H₂O₂ and YY and its every separated formulas respectively 16 hours later , the expression level of PLC-γ₁, PKCα and PLD were measured by Western blot analysis.Results; 1. YY and its every separated formulas can protect PC12 cells injured by H₂O₂, but the morphologic change in all groups is not significantly different. 2. YY and its every separated formulas can increase the survival rate of PC12 cells injured by H₂O₂, in which YY' sis the strongest. Subsequently to their effects on protecting PC12 cells injured by H₂O₂, the result is: YY> HG >HY>YG> H >Y>G. 3. After treated with YY and its every separated formulas , the protein expression level of PLC-γ₁, PKCα and PLD in the cells increased compared with the model group, the result is: YY> HG >HY>YG> H >Y>G.Conclusions: 1. YY and its every separated formulas can protect PC12 cells injured by H₂O₂ , and the three herbal medicines in YY have synergistic effects. 2. Oxygen-derived free radidicals may injure neuron cells via inhibiting PLC-γ₁—PKCα—PLD signal transduction pathway. 3. YY and its every separated formulas may protect PC12 cells injured by H₂O₂ via activating PLC-γ₁—PKCα—PLD signal transduction pathway. 4. Yang deficiency and Yin deficiency are the main TCM syndromes in neurological diseases caused by oxygen-derived free radidicals;simutaneously, Yang deficiency is more important than Yin deficiency. 5. Yang deficiency and Yin deficiency have close relationship with the weakening of PLC-γ₁—PKCα—PLD signal transduction and its molecular expressions in PC12 cells.