| Objective: The aim of this study was to investigate the expression of PIG11 (p53-induced gene 11) protein in human normal tissue and corresponding tumor tissue, discussing the distributing characteristic of PIG11 protein in human normal tissue and its function in the tumorigenesis and development.Methods: The expression of PIG11 protein was determined by immunohistochemical peroxidase-conjugated streptavidin (SP) method in 319 cases of human normal tissues and corresponding tumor tissues. And its expression was determined by the same way in human embryo hepatocytes L-02 and three human hepatocellular carcinoma cell lines Bel-7402, SMMC-7721 and HepG2.Results: It was positive expression of PIG11 protein in human liver, gastric mucosa, intestine mucosa, galactophore glandular epithelium and squamous epithelium tissue. Expression of PIG11 protein in cervical part of normal gastric mucosa gland was lower than that of its caudomedial part and bottom. Its expression was negative in human fibre, fat tissue, smooth muscle, lymph tissue and brain tissue. Expression of PIG11 protein was descent in liver cancer, poorly differentiated stomach adenocarcinoma, poorly differentiated intestine adenocarcinoma, breast cancer, comparing with those of corresponding normal tissues (p<0.01), and its expression was low in squamous carcinoma too, comparing with normal squamous epithelium (p<0.05). At the same time, the PIG11 protein of HepG2 cell line was down-regulated, contrasting with L-02 cell line (p<0.01).Conclusion: There was tissues specificity to the distribution of PIG11 protein in human normal tissues, and its expression was involved in cellular differentiation. It was distributed mostly in human epidermic tissues, including liver, gastric mucosa, intestine mucosa, galactophore glandular epithelium, squamous epithelium tissue, and so on, while, it was negative in human fibre, fat tissue, smooth muscle, lymph tissue and brain tissue.The... |
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