Bone Marrow-derived Flk1 ~ + Mesenchymal Stem Cells On Renal Ischemia Reperfusion Injury Repair And Its Inhibition Of Fibrosis Formation Mechanism Study,

Mesenchymal stem cell is a subpopulation of adult stem cells with powerful potential of self-renewal and multiple differentiation. A lot of studies on treatment of acute ischemia-reperfusion renal injury with mesenchymal stem cells showed that MSCs transplantation was doubtless effective in kidney recovery from acute renal injury, although there was still disputed on the mechanism of MSCs treatment. In this study, we study the effect of MSCs transplantation on acute tubular injury and subsequent fibrosis in unilateral acute renal ischemia-reperfusion injury model. Flk1~+ MSCs from EGFP transgenic mice were injected through tail vein after injury. Results showed that donor derived cells were engrafted into tubule and interstitial area on day 3 after MSCs transplantation. Collagen accumulation in MSCs treatment group was obviously less than that in control group by masson trichrome staining. Compared with control group, activated fibroblasts with α-SMA expression in MSC treatment group were distinctly reduced. RT-PCR was performed to analyze the level of inflammatory cytokines which were closely related with fibrosis development in kidney. The RT-PCR results showed that MSCs obviously inhibited the secretion of cytokines, such as TGF-P and PDGF. In conclusion, our study suggested that Flk1~+MSCs have therapeutical effect on acute renal injury. The possible mechanism included...