Pdgf-d Gene Polymorphism Associated With Stroke And Functional Study

Stroke is a major disorder which leads to mortality and morbidity all over the world. Stroke not only poses grave threat to human healthy, but also brings great economic burden to all countries. According to recent studies, the stroke incidence in China was the higher region all over the world, and the morbidity is increasing. The age-standardized annual death rate from stroke was 2 to 3 times higher than that from ischemic heart disease in China, whereas ischemic heart disease-related mortality is 3 to 4 times greater than the stroke-related mortality in Western nations. Moreover, the incidence of hemorrhagic stroke was 2 to 3 times higher in Chinese than in whites. Such differences might result from various environmental risk factors or different genetic backgrounds between the 2 populations.Ischemic stroke and hemorrhagic stroke share the common pathogenesis-atherosclerosis. Ross considered that atherosclerosis is an inflammatory disease. Platelet-derived growth factor D(PDGF-D) play an important role in the pathogenesis of atherosclerosis. Since stroke is a complex disease resulting from many risk factors, such as environmental and genetic risk factors. Single nucleotide polymorphism (SNP) techniques have supplied new methods to answer these complex genetic diseases. Since numerous studies have showed that PDGF-D involved in the pathophysiology of atherosclerosis, we hypothesis that PDGF-D may also related to the pathogenesis of stroke. In this study, we investigated the association of PDGF-D gene -858A/C and +3166C/G polymorphisms with stroke in Chinese Han population in a case-control study.In this study, 200 hundred stroke patients and 132 controls were recruited from 6 medical centers in China. Genotype was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) assay. Logistic regression analysis was performed to identify the independent risk factors for stroke.