Schistosoma Japonicum Recombinant Multivalent Vaccines And Immune Protection Function Assessment

Objective:Schistosomiasis is one of the most serious parasitic diseases, The research on Vaccine is an important and hard problem to the prevention and control of the disease in the world. The aim of this research is to investigate the coordination effecting protective immunity in mice by connecting LHD-Sj23 with the epitopes of Sj28GST and SjGCP to form multi-valent DNA Vaccines or multi-valent antigen vaccines. To expect find some higher protective Vaccines by this means. Methods: The epitopes of SjGCP was analyzed and elected by bioinfomatic method , The DNA for epitopes of Sj23, Sj28GST and SjGCP were amplified by polymerase chain reaction (PCR), these DNA fragments were insert in eukaryotic expression vector pCMV-script and prokaryotic expression vector pGEX-2T respectively with various combination forms. The recombinant plasmids pCMV-BSj23-BSj28 , pCMV-BSjGCP-BSj23, pCMV-BSjGCP-BSj23-BSj28 was extracted , purified and inoculated into Kunming mice by intramuscular injection. The recombinant prokaryotic expression plasmids pGEX-BSj23-BSj28 , pGEX-BSjGCP-BSj23, pGEX-BSjGCP-BSj23-BSj28 were induced by IPTG and expressed aim proteins successfully, these recombinant antigens were purified and inoculated BalB/c mice by hypodermic. Mice were attacked by Schistosoma japonicum cercariae. The infected mice were killed and the number of the adult worm was counted after 42 days of treatment.Results: The worm reduction rates from the three recombinant DNA vaccines pCMV-BSj23-BSj28, pCMV-BSjGCP-BSj23, pCMV-BSjGCP-BSj23-BSj28 were 6. 30%, 14. 76%, and 64. 95% respectively; The worm reduction rates from the three recombinant antigen vaccines expressed by pGEX-BSj23-BSj28, pGEX-BSjGCP-BSj23, pGEX-BSjGCP-BSj23-BSj28 were 13.65%,15. 7%, 57.99% respectively. Conclusions: The three recombinant DNA vaccines and the three recombinant...