| BackgroundThe prevalence of ischemic heart failure remains markedly high despite several recent therapeutic advances in the treatment of acute myocardial infarction. Alternative pharmacological treatments are being developed for the failing heart, but the mortality and quality of life in advanced stages of congestive heart failure are still significant issues. Recently,stem cell- based therapy has emerged as a promising treatment of severe postinfarction systolic left ventricular dysfunction.Because bone marrow mesenchymal stem cells (BMSC) are a population of the stem cells residing in the bone marrow other than hematopoietic stem cells. They have the ability of self-renewal and can differentiate into many functional cell types under proper environment.Another proposed effect of transplanting stem cells involves augmentation of angiogenesis and consequent improvement of myocardial ischemia.Howere, it is evident that transplanted stem cells do not survive well with, for instance, about 95%-98% of BMCS injected into the left ventricle of adult mice dying within 4 days of injection. Just these survival cells result in increased vascularity, reduced ventricles remodeling and improved cardiac function,but the mechanism is not known.In this study, BMSCs were treated in hypoxia and SD system in vitro to mimic conditions under ischemia. Then study the paracrine action of BMSCs on cardiac fibroblast cells (CFBs).ObjectiveIn vitro, BMSCs were exposed to culture condition that mimicked ischemia (hypoxia and serum-- free medium) over different periods of time and study the effection of rat MSCs-conditioned media (rMSCCM) on proliferation and collagen synthesis of CFBs. To further examine the role of paracrine mechanisms that underpin their therapeutic potential.Methods... |
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