| Objective:Radix Polygalae is the root peels of a Polygala tenuifolia family perennial herb Polygala tenuifolia Wild or ovum leaf Polygala sibirica. It can tranquilize the mind and eliminate phlegm. If it used improperly, Patients can be addressed to the stomach discomfort and nausea vomiting. Earlier research shows that the acute toxicity of Crude Radix P is high. It has an inhibition effect for stomach and intestine. Moreover, the research also show that the honey-stir-roasted Radix Polygala tenuifolia Willd has the same drug action of tranquilizing the mind and eliminating phlegm but has the less toxic. On this basis, we study further to investigate the acute toxicity of the constituents so as to confirm the toxic substance of Crude Radix P. We study to compare the constituents between Crude Radix P and honey-stir-roasted Radix P and search for the reason why honey-stir-roasting can release the toxicity of the crude Radix P .We study to inspect the influence on gastrointestinal motility of Crude Radix P, its toxic substance and honey-stir-roasted Radix P, so we can understand their influence on the target organ toxicity of the stomach and intestine. In addition, we study initially on the disposition of the target organ. Total target:Provide scientific basis for the explanation for the reason why honey-stir-roasting can release the toxicity of the crude Radix P.Methods:By chemistry methodology primarily, we use multi-disciplinary knowledge including chemistry -toxicology -pharmacology and so on to unify the research pattern, and conduct following several aspects research: 1. The saponins, the alkaloids, the xanthones and the fatty oils are extracted with the organic solvent and we investigate the acute toxicity and the tolerance doses of these constituents. 2. Using TLC, we analyze the difference on contents of sapogenin between the water-soluble extracts from the crude and the honey-stir-roasted Radix P. 3. We analyze the difference in the HPLC fingerprints of the water-soluble extracts from the crude and the honey-stir-roasted Radix P, and establish the fingerprint for the quality-control standard of the honey-stir-roasted Radix P. 4. Using methyl orange colorimetry of gastric emptying and charcoal propulsion of small intestine, to research the organ toxicity on stomach and intestines of Crude Radix P, its toxic substance and honey-stir-roasted Radix P. 5. Using spectrophotometriy, we try to determine the rudimental toxic substance in gastric juice after the mice are given crude and the honey-stir-roasted Radix P.Result:1. The median lethal dose of the saponins was 1.36±0.27g/kg. It was 5.88± 1.27g/kg when converting into the former medicinal materials. We could not calculate the median lethal doses of the alkaloids, the xanthones and the fatty oils, so we evaluated the tolerance doses of these constituents. The mice did not die when the dose was 500 times than people use. 2. The content of sapogenin in the water-soluble extracts from the crude Radix P was significantly higher than it in the water-soluble extracts from the honey-stir-roasted Radix P. (P <0.01) .3. The similarity between the fingerprints of the water-soluble extracts from the crude and the honey-stir-roasted Radix P was less than 0.95, and then they had the certain differences. 4. Toxicity of... |
