| Objective:To observe the preventing and treating effect and explore the function mechanism of Gankang IV(GKIV) on fatty liver to provide the experimental basis for the clinical therapy of fatty liver. Methods:64 wistar rats were feed with feedom food and water in 20-23 ℃ the commonness clear animal laboratory for a week,then divided randomly into model group(n=14),GKIV low dose group(n=10),GKIV medium dose group (n=10), GKIV high dose group (n=10) , Dongbaogantai (DBGT) group(n=10) and normal control group (n=10). From the first day of the experiment each group animal was feed with high fat food and Zhijiangdaqu (the alcohol content 45%) 1ml/100 g/d to fill stomach at 9: 00 A. M except the normal control group, which was given common food and 0. 9% sodium chloride lml/100 g/d. The model group and normal control group were filled with physiology brine 1ml/ 100g/d at 4: 00 P. M, while GKIV low dose group,GKIV medium dose group,GKIV high dose group were done with concentrate liquor of GKIV 1ral/100g/d (the respective druggery content: 0. 5g/mk 1.0g/ml, 2.0g/ml), DBGT group was done with dongbaogantai 1ml/100g/d (the druggery content 8. 8mg/ml). From the end of NO. 4 week, one of the model group was killed randomly and the liver was taken to make pathologic check for observing the developing of making model. At the end of NO. 8 week, all animals were forbidden food and water in 24 hours and anaesthetized by celiocentesis with 10% Chloral Hydrate liquid 0. 2ml/100g to weigh them. Then the eyeballs were extirpated to take the blood 2ml and check the liver function immediately. Small pieces of hepatic tissue at the edge 0. 5 cm distance of liver biggest leaf were taken ,flushed in ice physiology brine and blotted with the filter paper, taken 0. 5g to be placed in 5 ml ice physiology brine, and squeezed quickly by the inside slice type grinding machine 10000 r/min 10 seconds/time (continuing 3 times, interval 30s) to be maked into the 10% hepatic tissue fluid, then placed in 4 ℃ the high speed refrigeration centrifugal machine and turned 3000 r/min for 10 minutes. At last taked the fluid 3ml and sealed completely -20 ℃ to save until check. 3 pieces of hepatic tissue in different part ( 1 × 1 × 0. 5cm)were extracted, soaked in 10% formaldehyde solution to fix, sliced, then maded HE staining and observed morphological changes by optical microscope. Results:1 The liver cubage of model group augmented obviously, yellow, envelope tension, edge blunt, texture soft by naked eye observation. The normalstructure of liver was changed . There were many different fat vacuoles in most liver cells and appeared medium or serious steatosis (fatty degeneration) , liver lobule and liver antrum were not clear by optical... |
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