Tetramethylpyrazine Fetal Growth Restriction And Insulin-like Growth Factor Ii Expression

Objection Analysis the variation of IGF- II level on the FGR rats , fetal length , fetal weightand fetal Poundera Index and inquiry into the effect of Ligustrazine, to offer the theoretical basefor treating FGR with Ligustrazine.MethodsThe model of FGR rats was made by means of passive smoking and drinkingalcohol.The rats were divided into control group, FGR model group and Ligustrazinegroup. Some pregnang rats were treated with Ligustrazine group.At day 20of gestation,fetal ratswere delivered by cesarean section. Fetal weight, length were measured and compared. SerumIGF-Ⅱ concentrations were measured by radioimmunoassay(RIA);Placenta and Fetal liverIGF-Ⅱ expression levels were determined by immunohistochemistry(IH) and multimediacolorful patheological image analysis technique; Placenta was dyed with HE ;The ultrastructuralchanges of placenta of the delivered rats were observed by light-glass and electric-glass.Results (1) In three groups the fetal weight and length were significantly different (p<0. 05) :the fetal weight and length in control group> those in Ligustrazine group >those in FGR group.(2) The fetal Poundera Index in control group was higher than that in FGR group and in FGR+interference group (p<0. 05) , the fetal Poundera Index in Ligustrazine group was higher thanthat in FGR group ,but was not significantly different.(3)In three groups ,the concentrations of serum IGF- Ⅱ in rats were not significantly different;The fetal serum IGF- Ⅱ concentration were significantly different(p<0. 05): the concentrationsof serum IGF- Ⅱ in control group> those in Ligustrazine group >those in FGR group.(4) The villlus gap area's percentage in FGR group was higher than those in other two groups (p<0. 05) .(5)The placenta and fetal liver IGF-Ⅱ expression levels in three groups have significantlydifferent (p<0. 05) .Conclusions (1)The serum IGF-Ⅱ's source in rat is different from that in fetal rat ,andIGF-Ⅱ can not pass through the placenta.(2) The assay of the serum concentrations of insulin-like growth factor-2 in rats can not diagnose FGR.(3) The decrease of fetal serum IGF- Ⅱ concentration ,placenta and fetal liver IGF- Ⅱ expression levels may play an important role in FGR.(4)Ligustrazine can be effective for treatment of FGR.