| Objective: To evaluate the impact of transplanting bone marrow-derived mesenchymal stem cells (BM-MSCs) via noninfarct-relative artery in the pig myocardial infarction model. Method: BM-MSCs were obtained from pig bone marrow, expanded in vitro to the purity of >50%, and labeled with myocardial bromodeoxyuridine (Brdu). Myocardial infarction (MI) was created by ligating the distal left anterior descending artery in pigs. Either Brdu -labeled cells (5×10~6) or culture medium were transfused into infracted area via noninfarct-relative artery at four weeks after MI. Gate cardiac perfusion imaging and coronary angiography were performed at the same time and four weeks after cells transplantation. Morphologic and histologic characteristics of the heart were also studied. Result: Survival cells labled with BrdU can be identified in host myocardium, which demonstrated expression of myosin heavy chain p. BM-MSCs transplantation groups had a greater (P < 0.05) capillary density than did the control group. The gate cardiac perfusion imaging showed that the heart function with the cell... |
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