Probiotics Natural Isolates H24 Of E. Coli O157: H7 Infection Of Inhibition Study

Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for outbreaks of bloody diarrhea (HC) and hemolytic-uremic syndrome (HUS) in many countries. Traditional treatment of infectious diseases is based on compounds named antibiotics that aim to kill or inhibit bacterial growth. Vital systems of the bacterial cells are the drug targets of these types of compounds. As a consequence, the bacterial SOS response, a ubiquitous response to DNA damage, is induced. Evidences showed that toxin synthesis by EHEC appears to be coregulated through induction of the integrated bacteriophage, also called prophage, 93 3v and 93 3w which encode the toxin gene stxl and stx2 respectively. And prophage production is linked to induction of the bacterial SOS response. So the ultimate conclusion was that the SOS-inducing antibiotics was responsible for the SOS-mediated STX synthesis, and also could exacerbate EHEC infections in clinical practice. So, it will make sense to screen antimicrobial agents or probiotics for the prevention and control of O157:H7 infection to some extent. Our study aimed to discuss the feasibility of the control of E. coli O157.H7 employing Bacillus sp. as the role of probiotics.The discovery of bacterial-communication systems (quorum-sensing systems), which orchestrate important temporal events during the infection process by secreting a type of chemical molecules named autoinducer (AI), has afforded a novel opportunity to ameliorate bacterial infection by means of interfering or interrupting the communication between each of the pathogenic microbes other than growth inhibition. This system is considered as a target for antimicrobial therapy by more and more scientists now.The application of probiotics was considered as an indispensable approach to inhibit enteropathogenic microorganism infection. The strain H24 discussed in this study was isolated in the fonner work, and the preventive and therapeutic function of H24 to gallinaceous, swinish and bovine diarrhea was described. This strain was identified as Bacillus amyloliquefaciens in this study. A common thread holding together much of our work was the melding of Quorum-sensing hypothesis and research of probiotics. The natural products of H24 were fermented, and the...