Model Of Hepatic Steatosis Influence Mechanism Of TNF-¦Á And Insulin On The Model

Objective To establish hepatocyte steatosis models induced by the effect of different concentration oleic acid,palmitic,the mix of oleic and palmitic acids, and ethanol on hepatocarcinoma cell HepG2 respectively.Methods The cell model was established by treating human hepatocarcinoma cell stain HepG2 with fatty acids and ethanol.The optimum concentration of fatty acids and ethanol should be definited by MTT colorimetry test .After the cells were treated for some time ,the fatty droplets in cells could be observed by Oleic red O staining .The intracellular triglyceride was detected by enzyme coupling colorimetry after using cell splitting fluid. The leakage ofγ-Glutamyl transpeptidase(γ-GT) in medium was detected by heavy nitrogen reagent colorimetry.Results After treating HepG2 cells with 0.05mM,0.10mM,0.25mM,0.35mM oleic acid or 0.10mM oleic plus 20U palmitic,0.25mM oleic plus 10U palmitic for one day , and 5U,10U,20U,30U palmitic acid or 0.6%,1.2%,1.4%,1.6% ethanol for four days ,all led...