Prevention Of Helicobacter Pylori Infection And The Development Of The Egg Yolk Antibodies

Objective: To observe anti-rHpaA IgY from eggs yolk of hens immunized with recombinant Helicobacter pylori HpaA protein by gene-engineering.Methods: Recombinant Helicobacter pylori HpaA protein were aquired by gene-engineering. Laying hens were immunized with recombinant. rHpaA.IgY was isolated by using the water dilution method (WD) ,and their concentration and purity were measured respectively with Bradford method and SDS-PAGE. The specificity of IgY and titer of IgY was assayed with Western blot and ELISA respectively. The titer of post-pasteurized was assayed with ELISA.Results: The concentration and purity of IgY were 24.6mg/ml and 90 % respectively. The titer of IgY was 1:12800,and the titer of post-pasteurized did not decrease. Its specificity was confirmed by Western blot.Conclusion: rHpaA-IgY with high concentration, high purity, high titer, pasteurized-stability and specificity was successfully preparated.