Gliquidone Impact Of The Islet Of Hit-the T15 Cell Insulin Secretion And Oxidative Stress

PART ⅠGliquidone features different from glibenclamide in the insulin secretion pattern of HIT-T15 cellsObjective: This study aims to determine whether gliquidone has any different effect on the insulin-secretory profiles of HIT-T15 cells from the other two sulphonylureas i.e. gliclazide and glibenclamide in response to glucose stimuli.Methods: HIT-T15 cells (β-cells line) were cultured for 8h in RPMI-1640 with three different glucose concentrations (5, 10, 15mmol/l), the cells were re-cultured with gliquidone ,gliclazide and glibenclamide in various concentrations ,and then, insulin contents in the medium at 0, 10, 20, 30, 45, 60, 120 and 180 minutes were measured by radiommunoassay(RIA). Results: In this study, all three sulpholureas strongly stimulated the hormone release in the present of glucose in different patterns by the β -cells. Gliquidone and gliclazide induced a similarly rapid biphasic insulin secretion, reaching the first peak at 10 minutes and the second lower peak at 30 minutes, followed by gradual decline to near the basal values at the third hours. In contrast ,glibenclamide induced a strong but monophasic insulin release, reaching its peak at 45 minutes followed by somewhat decline of the hormone release but didn't return to basal values in 3 hours later. Conclusion: Gliquidone stimunites insulin release by HIT-T15 cells in a way different from glibenclamide, but similar to gliclazide. This unique feature in insulin secretion of gliquidone may have important implications in its clinical...