Transgenic Lycium Expression To Hiv Recombinant Capsid Protein Identification And Immunogenicity Of The Preliminary Study

With the potential value for inexpensive production of high-quality proteins for commercially valuable and clinically purposes, the bioreactor of transgenic plants has been gaining the increasing awareness. Plant-based production systems have more advantages than prokaryotic and animal cell expression systems. On one hand, the plant cell produces much safer proteins than prokaryotic and animal expression systems, such as: plants are free of animal pathogens, no potential for contamination with animal disease agents. On the other hand, the target protein from plant cells could be purified easier than from other expression systems, especially some cost-effective methods for the tissue of the leaves and seeds of the transgenic plants. It was estimated that the production costs for foreign proteins are at least one to hundred-fold less in plants than in other expression systems. Furthermore, transgenic plants propagate much more rapidly and can be scaled-up in extremely short time, giving hundreds of seeds. Finally, plant systems have been shown more similar post-translational modification to those in animal cells. It was reviewed that full-length mammalian proteins produced by plant systems appear to be similarly processed to their native counterparts and even assemble into quaternary structures, for glycosylation patterns, there are much little difference between plant and animal systems.Acquired immunodeficiency syndrome (AIDS) remains a serious global health problem, with the number of HIV carriers exceeding 58 million worldwide, and young people (15-24 years old) account for nearly half of all new HIV infections worldwide. As an important early marker of HIV infection, the HIV-1 CA capsid protein is a good candidate for the HIV vaccines, for inducing cellular and humoral immune responses. MA–CA junction had been shown to play a important role in the fusion proteins assembly into spherical particles, especially the last four amino acids of the Matrix, unlike the protein CA assembly into cylinders in vitro.It was detected that the synthesis of the CA immunodominant epitope protein in transgenic tobacco plants. To enhance the cellular and humoral immune responses to CA, we using the constructed plant expression vector containing the CA gene encoding a 151-aa fussion peptide that could induce cellular and humoral immune responses. The larger antigen protein could generate additional VLPs that may protect...