Extraction And Separation Of Taxol And Its Analogues In Yew Foliage And Determination,

Taxol is a kind of terpene compounds, which was separated from Taxus brevifolia originally. Because of the special anticancer mechanicsand special curative effect for some kinds of tumor, Taxol attracts much attention recently. It is very difficult to separate and purify Taxol from Taxus, because the content of the Taxo1 in Taxus is very low, and there are also some analogues of Taxol in Taxus , which make it more diffeult to separate.Consequently, it is very important to develop a method to separate and purify Taacol with high recoveries.This thesis includes five parts.The first part includes the review of the latest researches on Taxus, the studies on the extraction and purification of taxol; the studies on the antitumor effect of taxol; the determination of residual volume of the organic solvent in the drug and the problems met in the manufacture procedures,and 113 references were cited.The second part is about studies on the extraction technology of Four Tank Counter Current to extract taxanes from Taxus. We extracted taxol and analogs from Taxus with the method of Four Tank Counter Current, and investigated in detail the factors which may affect the technology, including methanol concentration, the contact time, the temperature and so on. And then we determined the best condition and established a new extraction technology of taxol.In the third part RP-HPLC was used to determine the contents of taxol in the extract of Taxus, and a method used to determine the contents of taxol in the extract of T. Canadensis Leave was established, while the results showed that this method was easy and accurate and had good precision. Then this method was adopted to determine the contents of taxol in the extracts of different kinds of Taxus. In the fourth part HPLC and RP-HPLC was combined to establish a new method of extracting Taxol, Cephalomaninne, 10-DAB,Taxinine,Taxagifine and 7-epitaxol from the Taxus extract. A new homemade porous polymer resin filling (JTY-1) was applied as stationary phase in the RP-HPLC. The results of the experiment showed that JTY-1 showed high selectivity towards taxol and cephalomaninne, and had small irreversible adsorption, and what's more, this kind of filling is reclaimable.The last part is about the determination of residual volume of the organic solvent in the pure taxol. Headspace Capillary Gas Chromatography and programmed temperature were applied to determine the residual volume of acetone and n-hexane in the taxol. The results showed that this method was accurate and sensitive, and suitable for the determination of residual volume of acetone and n-hexane in the taxol.