Objective :1. To study the corelation between the mobidity of asthma and inflammatory cells expressing of TGF-β1 and IL-5 in sputum 2. To explore the feasibility of immunocytochemistry assay with sputum specimens obtained by vacuum aspiration.Method:sputum specimens of 39 cases were obtainted by vacuum aspiration, asthma group(acute asthma with pneumonia):24 cases, non-asthma group(acute pneumonia):15 cases. The airway interrupter resistance(Rint) of all cases were measured with Microloop spirometry device. 2. The sputum specimens were centrifuged after DTT management, and the supernatant preserved at -70℃ was assessed for content of IL-5 with ELISA. 3. The depositor was smeared for Eos count and measurement of TGF-β1 expression of inflammtory cells. Result:1.The mean Rint was significantly higher in asthma group (0. 53 ± 0. 26) kPa/L · S than in non-asthma group (0.25 ± 0.16) kPa/L · S, P<0. 01. The Eos count and expression of IL-5 were higher in asthma group than in non-asthma group , (P <0. 01, P < 0. 05) respectively. 2. 15 cases showed positive expression of TGF-β1 of inflammatory cells in sputum of asthma group, there is no positive expression of TGF-β1 in non-asthma group, Forthermore, the ratio of TGF- β1 positive expression showed linear correlation with Eos count and IL-5 (r=0. 780, P<0. 01,r=0. 892, P<0. 01) respectively. There is no relationship with the Rint.Conclusion: 1. The over-expression of IL-5, Eos count and TGF-β1 of inflammatory cells in sputum of asthma patients reflects the affection of asthma patient' s airway, which may provide evidences for diagnosing and treatment by further studing. 2. Sputum from infantile asthma patients can be taken for immunocytochemistry assay, but the experiment condition should be optimized. |