Since The Proposed Side Suppressor Soup On Nci-h446 Human Lung Cancer Cell Proliferation In Vitro Experiments

Objective: The topic cell culture technology, Since the adoption of the proposed side suppressor soup on human lung cancer cell line NCI-H446 interference, explore its proliferation. From seedling to the Chinese Medicines board prescription drugs found lung cancer cell proliferation inhibition new method for the treatment of lung cancer with a new treatment ideas.Methods: NCI-H446 cells were cultured in plotting NCI-H446 cells growth curve, from the log at the NCI-H446 cells for experiments. First determine the role of tumor suppressor soup concentration of tumor suppressor soup into 1g/ml,100mg/ml.10mg/ml,1mg/ml,100ug/ml,10ug/ml, 1ug/ml, 0.1ug/ml a total of eight concentration gradient, the control group only increase contour cell culture media, a total of nine. Each group set up four Minute hole, MTT assay was used to measure cell survival. Cells from the 50% survival rate for the drug concentration as a tumor suppressor soup administrationb reference concentration. In further unrest 20ug/ml,40ug/ml,60ug/ml,80ug/ml suppressor, 100ug/ml 5 concentration and 24 hours, 48 hours, This 72-hour observation time three point blank (media control group) were compared observation. For the final soup 60ug/ml suppressor as the concentration of tumor suppressor soup administration concentration, and the Western group. suppressor Tonga medicine group (60ug/ml concentrations and increases with western medicine) and the only culture fluid of the control group. A total of four larger groups (suppressor of group, medicine group, the tumor suppressor Tonga medicine group, the control group), a group set up four of the Minute hole, Comparative study, the MTT assay was used to measure the cell growth rate, the above experiments were repeated three times.Results:1 : Drawing cell growth curve inverted microscope for cell counting, cell doubling time was 26 hours. 2 : NCI-H446 tumor suppressor soup of cytotoxic cell experiments at the 50% survival rate of drug concentration in the range of 1-10 0ug/ml between, according to the concentration range for the inhibition of cell proliferation with reference to the concentration of drug administration. 3 : inhibition of cell proliferation : the tumor suppressor 20ug/ml,40ug/ml,60ug/ml soup, 80ug/ml,100ug/ml concentration and 24 hours, 48 hours, This 72-hour observation time three point blank Group (media control group) were compared statistically significant difference. Suppressor soup three experimental groups after statistical analysis : P=0.492. suppressor soup that is repeated three experimental groups no significant difference on experiments with repeatability. The results showed that different concentrations of tumor suppressor role of soup for 48 hours NCI-H446 cells was inhibited, With the duration of effect gradually weakened, and with the dosage increased, the inhibition. Suppressor soup in 24 hours, 48 hours, 72 hours of inhibition rate reached 53.47%, respectively, 54.47% and 10.45%.4 : four administration of comparisons between groups : Group selected tumor suppressor soup in the middle concentration (60ug/ml). Suppressor Tonga medicine group and tumor suppressor soup; Suppressor Tonga western medicine and western medicine; Suppressor soup and western medicine; suppressor soup group and control group; suppressor Tonga medicine group and control group; Western and blank cells inhibition rate was statistically different. Through the administration of several groups were compared on February 2 : suppressor Tonga medicine group than suppressor soup; Suppressor Tonga better than Western medicine group; WM group than suppressor soup; suppressor soup is better than the blank group; suppressor Tonga medicine group is better than the blank group; Western medicine is better than the blank group and the significant difference.Conclusion: 1 : human small cell lung cancer NCI-H446 cells doubling time of 26 hours, rapid proliferation. 2 : The results showed that different concentrations of tumor suppressor role of soup for 48 hours NCI-H446 cells was inhibited, With the duration of effect gradually diminish. 3 : soup of NCI-H446 tumor suppressor cells with the dosage increased, the inhibition. 4 : Tonga medicine group suppressor tumor suppressor than soup; Suppressor Tonga better than Western medicine group; WM group than suppressor Soup. Suppressor unrest in vitro human small cell lung cancer NCI-H446 cells were inhibited by Shugan Jimmy gas, Huoxuezhitong, and removing expectorant, Qingrejiedu inhibition of cell proliferation. Have potential medicinal value, it is worth further study.