The Nature Of Drugs And Proteins In The Amino Acid / Surfactant / Water System

1. The Properties of Drugs and Human Serum Albumin in L-Glutamic Acid/1-Aminodecane/H₂O (GC₁₀/H₂O) SystemThe critical micelle concentration of L-glutamic acid/1-aminodecane (GC₁₀) has been measured by the methods of surface tension and conductivity. The interactions among GC₁₀/H₂O, ibuprofen (Ibu) and human serum albumin (HSA) have been studied by UV-vis spectrum, fluorescence spectrum. The morphology behaviors of between GC₁₀/H₂O/SDSsystem and GC₁₀/H₂O/Ibu system, the effect of GC₁₀ on the structure of HSA have been studied with negative-staining transmission electron microscope. The results show that vesicle and microphere can be formed in GC₁₀/H₂O/SDS system. The interaction between GC₁₀ and ibuprofen can improve the solubility of Ibu. Meanwhile, the structure of Ibu has not been changed. Ibu can exist in the microsphere in GC₁₀/Ibu/H₂O system. At different pH values in GC₁₀/Ibu/H₂O system, the morphology behaviors of the mixted system are different. The interactions between GC₁₀ and HSA, the interaction time all have some effects on the spectrum properties of HSA. The properties of acyclovir (Acy) and HSA also have been studied.2. The Properties of Ibuprofen (Ibu) in Amino Acid/Dodecylamine (DA)/H₂O SystemThe bebaviors of amino acid/amine/H₂O mixed system and Ibu have been investigated by the methods of UV-vis spectrum, fluorescent spectrum, isothermal titration calorimetry (ITC) and negative-staining transmission electron microscope. The mixed system of amino acid/amine/H₂O can improve ibuprofen solubility. Ibupfofen in the mixted system is encapsulated by vesicle or the particle size of ibuprofen decreases and even forms microshpere. The mechanisms of microsphere and vesicle formation in His/DA/H₂O/Ibu are similar to that in Glu/DA/H₂O/Ibu systems, but different from that in Gly/DA/H₂O/Ibu system. A large number of vesicles is formed in Gly/DA/H₂O system with the increase of Ibu concentration. Ibu can be encapsulated gradually by vesicle, and finally exist in the microshpere in Gly/DA/H₂O/Ibu system. The interaction of amines, with different carbon chain length, and Glu, SDS can induce the morphology change of SDS aggregate.3.The Properties of Drugs and Hemoglobin in N-Methyl-D-glucamine (MEG)/H₂O SystemThe surface activities of N-Methyl-D-glucamine (MEG) and MEG/PEG6000 have been studied using surface tension and conductivity methods. The interactions of MEG with ibuprofen are also studied by the methods of UV-vis spectrum, fluorescence spectrum, ITC and negative-staining transmission electron microscope. The results show that both MEG/H₂O system and MEG/PEG6000/H₂O system can greatly improve ibuprofen solubility. A large number of vesicles can be formed in the systems. Ibuprofen is directly integrated in the bilayer of vesicle and finally encapsulated. Added pyrene probe to MEG/H₂O/Ibu system, ibuprofen can drive the probe into the hydrophobic inner cores of the microsphere and vesicle, which cause the micorpolarity change of the probe. The systems may accelerate the agglomerate of hemoglobin (Hb) molecules. When MEG concentration is higher than 5.0×10-5 mol?L-1, the interactions of MEG, Hb and drugs have some significant effects on the properties of Hb and drugs.