Dynamic Observation Of The Enterococcus Faecalis Biofilms And Drug Sensitivity Studies

Goal: But the domestic and foreign correlation research report confirmation, the excrement enterococcus is the root canal backfill consummates the treatment defeat main microorganism reason, and mainly by biomembrane form field planting in infection root canal tooth essence wall.At present the massive experiments determines the medicine to the suspended state excrement enterococcus resist bacteria potency, this topic altogether focuses microscope (CLSM) using the laser and draws support from the fluorescence microscope and the ordinary light microscope observes in vitro excrement enterococcus biomembrane the dynamic forming process, the key comparison studied the calcium hydroxide with to wash must the peaceful two kind of antibiotics to the biomembrane condition excrement enterococcus sensitivity, after was for the purpose of discussing two kind of root canal medication function to form the excrement enterococcus biomembrane again the influence effect, the revelation biomembrane has the formidable antibiotic nature compared to the aerosol fungus, for on the clinical active control excrement enterococcus infection enhanced the success ratio which the root canal treatment and the non-surgery treated again to provide the theory basis.Method: Tests a use the biomembrane model which produces on the slide, pre-experimental ordinary light microscope observation excrement enterococcus biomembrane forming process; (Molecular Probes L7012 USA) carries on the dyeing with LIVE/DEADBacLight Bacterial Viability Kit to the biomembrane bacterium, will dye after the biomembrane to put in under CLSM to observe the biomembrane the internal structure, quantitative investigation biomembrane thickness, the inner layer, the intermediate level and the outer layer bacterium density and the bacterial activity, and has made statistics analysis.Tests two first uses two time of dilution method determinations to wash must peaceful to excrement enterococcus smallest bacteriostasis density (MIC) and smallest sterilization density (MBC), then altogether focused microscope (CLSM) using the laser and draws support from the fluorescence microscope to compare has washed must peaceful and the calcium hydroxide two medicines to the excrement enterococcus biomembrane function effect, again used to raise 24h the mature biomembrane to make the comparison normally, after further used the statistical method quantitative analysis medicine function magain to raise 24 h biomembranes in the bacterium density and the active difference. Finally: Tested one had initially determined the biomembrane basically formed the time is 6h, the mature time was 24 h, observed the excrement intestines coccus biomembrane thickness change; In the membrane the bacterium mainly concentrates in the inner layer and theintermediate level, the outer layer distribution are less; In the membrane the live fungus mainly concentrates in the intermediate level, the inner layer and the outer layer are less; Washes must peaceful to the excrement intestines coccus smallest bacteriostasis density (MIC) and the smallest sterilization density (MBC) respectively be 0.03125%, 0.0625%; Observed the excrement intestines coccus biomembrane to 2% to wash must the Taihe calcium hydroxide (PH=10) the formidable antibacterial activeness, after namely the large dose antibiotics function, the biomembrane basic structure unit micro colony still existed fact; Washes must peaceful and the calcium hydroxide two kind of medicines after the excrement intestines coccus biomembrane function, then forms the biomembrane the bacterium density and the active existence difference.Conclusion: The biomembrane forms the time quickly, after it forms the internal structure stabler, in the membrane the bacterium mainly in the inner layer and the intermediate level, the live fungus mainly concentrates in the intermediate level, the outer layer fungus sparsely, also are many is the dead fungus; The biomembrane state excrement intestines coccus compared to floats the fungus to have the formidable antibiotic nature, after the large dose antibiotics function, the biomembrane basic structure unit micro colony stillexisted; Had proven after 2% wash must the peaceful function againform to the biomembrane has obviously delays the function.