Hlg Treatment Of Women With Rubella Virus Active Infection

Objective:To deploy clinic and empirical study, the purpose of this thesis isto observe the therapeutic effect and security of Huanglan Granula totreat reactive infection of rubella virus for women and to approach themechanism of rejection capability for rubella virus, for providingscientific evidence to clinic generalization and application.Clinical research:The cases we elected were all from the OPD of the No. 1 hospital ofWuhan between the March of 2005 to the May of 2006. The women were allchild-bearing age who had consulted for aristogenesis and fetation plan,and they were final diagnosed of RuV reactive infection by the way of ELISA.The subjects were randomly divided into treatment groupand control groupby using random digits table, 30 cases of each group. The treatment groupcases were given with Uuanglan Granula, one dose per day, which taken aftermixing with warm water twice daily. The control group cases were givenwith ribavirin, 0.2 gram per day, three times daily. Defined 20 days asone course of treatment, the RuV-IgM was re-examined 10 to 14 days afterdrug withdrawal, while continued to be treated if not turn to negative.The IL-2 and TNF-αof all cases were observed before and after treatment.The serum RuV-IgM of that turned to negative after treatment wascontinued to be monitored for 3 months. According to the data recorded,the statistical treatment was implemented.Empirical study:There are 3 steps in this experiment. 1. Detect the titre of RuVInfection: The rubella virus of different dilution was inoculated in Verocells, the cytopathic effect was observed and the TCID₅₀ was calculatedaccording to the formula. The virus of 100 TCID₅₀ was deployed to infectthe ceils in this anti-virus experiment. 2. Detect the cytotoxicity of drugs: The stock solution of drug with different dilution was inoculatedin Veto cells, the cytopathic effect was observed and the cell survivalwas detected with MTT. The median toxic concentration and the extent ofmaximum nontoxic concentration of guanglan Granula were calculated. 3. Theanti-virus experiment: According to the periodicity of virus replication,three groups were divided into: anti-virus adsorption group, directinactivation group and anti-virus biosynthesis group. In each group,ceils control group, virus control group and positive control group wasset up respectively. The anti-virus function of drugs was analyzed, thevirus inhibition rate was detected with MTT, the IC₅₀ and the TI wascalculated.Results:Results of clinical research: Among the 30 cases of treatment group, 26cases turned to negative after one course of treatment, the rate was 86.67％,while 19 cases of control group turned to negative, the rate was 63.33％.There was significant difference between the 2 groups (P＜0.05). All casesof treatment group turned to negative after two courses of treatment, therate was 100％, while the rate of control group was 86.67％. There was nosignificant difference between the 2 groups (P＞0.05). The serum IL-2concentration before and after treatment of treatment group was 83.87±39.93 pg/ml, 262.40±117.91pg/ml respectively. There was significantdifference between the numerical data (P＜0.01). The serum TNF-αconcentration before and after treatment of treatment group was 105.40±45.20 pg/ml, 55.30±18.46 pg/ml respectively. There was significantdifference between the numerical data (P＜0.01). The serum IL-2concentration before and after treatment of control group was 81.97±40.97pg/ml, 107.70±58.58pg/ml respectively. There was no significantdifference between the numerical data (P＞0.05). The serum TNF-αconcentration before and after treatment of control group was 99.03± 46.58 pg/ml, 80.17±33.19 pg/ml respectively. There was no significantdifference between the numerical data (P＞0.05).Results of empirical study: The TCID₅₀ of RuV GOS strain in this experimentwas 10^-4.22/0.1 ml. In the experiment of cytotoxicity, there was nosignificant cytopathic effect at the concentration of 0.16mg/ml, so theconcentration of 0.16mg/ml was determined as the maximum nontoxicconcentration. The IC₅₀ of Huanglan Granula to Veto cells was 1.71mg/mL,and the IC₅₀ of ribavirinwas 1.08mg/mL. In the experiment of anti-virus,the degree of cytopathic effect was attenuated and the virus inhibitionrate was increased with the concentration of drugs increased in theconcentration extent of 0.01,0.02,0.04,0.08,0.12,0.16mg/ml. Therewas direct correlation between the concentration of dugs and the virusinhibition rate. HuanglanGranula could not intercept the adsorption ofvirus and could not directly kill virus either, but generated antiviralactivity through inhibiting biosynthesis of virus. The IC₅₀ of UuanglanGranula to RuV was 0.10mg/mL, and the TI was17.1. The IC₅₀ of ribavirinwas 0.08mg/mL, and the TI was 13.5Conclusion:1. The therapeutic effect of Huanglan Granula to the reactive infectionof rubella virus is significant, and it can efficiently promote thespecific antibody of rubella virus turn to negative.2. There is immunologic injury effect when infected rubella virus, andthere is correlation between the concentration of IL-2 and rNF-αandthe extent of reactive infection. Huanglan Granula can repair theimmune function through recovering the concentration of IL-2 and TNF-3. Huanglan Granula has antiviral activity through inhibitingbiosynthesis of virus.4. Huanglan Granula is an effective Chinese drugs pharmaceutics for treating reactive infection of rubella.