| Bronchial asthma is a combination of cells (such as eosinophils, mast cells, Tlymphocytes, neutrophils, airway epithelial cells, etc.) and cellular componentsinvolved in the chronic airway inflammatory diseases. In recent years, on airwayinflammation, airway remodeling in endless, a major breakthrough is clear asthma is,in essence, the chronic airway inflammation. However, simply using the airwayinflammation can not fully explain bronchial asthma, People again as airwayremodeling in asthma research focus. Large autopsy information shows that patientswith asthma airway smooth muscle layer thickness than 50% above normal, Itsthickness is not just compensatory cells, mast results More is the main airway smoothmuscle cells due to the proliferation itself. ASMC proliferation of the ARM haveimportant reasons, with airway hyperresponsiveness are closely related. Therefore, theconventional anti-inflammatory treatment on the basis of research and developmentASMC inhibit the proliferation of drugs, BA likely to treat new and more effectiveapproaches.ObjectiveBy observation Xuanfei Dingchuantang-containing serum of the rats in vitroproliferation of ASMC. and to explore the mechanism of its action, to understandXFDCT in airway remodeling—ASMC an important aspect of the proliferation ofdirect role.Methods1. Clean a male Sprague-Dawley rats, spinal dislocation law were killed in asepticconditions, from the middle left lung, the right middle and lower lobes of the lungtissue fluid separated the bronchial. Will be separated into good beating bronchialtissue block size, with the original explant culture ASMC.2. Histamine (histamine. His) induced apoptosis in cultured airway smooth musclecells proliferation of cells.3. Clean 20 Wistar rats were divided into five groups: XFDCT high, medium and low dose group, Western and control groups, each group four. XFDCT high, mediumand low dose rats were given to the parties by Decoctions 40g/(kg·d), 20g/(kg·d),10g(kg·d), respectively, 20ml/kg. WM group to dexamethasone 2.54X10-4g (kg·d) volume suspension gavage; blank group to the same dose of saline wasadministered for five consecutive days. In the last administration before fasting for 12h, but could not help but water, the last hour after gavage. 10% chloral hydrate(1ml/kg) anesthesia, abdominal aortic blood, blood collection tube 4℃overnight.Serum full until precipitation 3000rpms/15min centrifugation, the collection of serum,56℃/30min inactivated complement,0.22um filter membrane-packing,-20℃standby.Respectively role in the group ASMC.4. Inverted microscope observation of cell morphology and growth conditions andradiography. MTT assay and cell proliferation immunocytochemical staining (SABC)in the detection of proliferating cell nuclear antigen expression.Resuls1. Through anti-α-actin irnmunohistochemistry staining,all of the cells'staining areMasculine,the purity coefficient 95%.2. His role in different concentrations in ASMC, in different time points werehigher than A570nm value, 10-4mol/L in which the concentration of ASMC His mostvisible role, 36h in the A570nm value of the control group was 1.19 times (0.152±0.002 compared with 0.1 28±0.003, P＜0.05,n=5). His right ASMC showedsignificant role in promoting proliferation (P＜0.05,n=5). Time-dependent relationshipexists but not a concentration-dependent manner.3. Serum containing high or middle dose XFDCT could decrease the OD value ofASMC tested by MTT method,and there was singificant difference (P＜0.05)comparedwith the control group,but there was not singificant difference(P＞0.05)compared withthe dexamethasone group. XFDCT low-dose group and the control group were notsignificantly different (P＞0.05). And there is a certain time and dose dependentmanner.4. PCNA located in the nucleus, the positive nuclei within the brown granules,MIAS2000 image analysis showed high-dose and middle-dose group PCNA expression in the the average optical density was significantly higher than the controlgroup (P＜0.05), there are significant differences; and dexamethasone group was nosignificant difference (P＞0.05). XFDCT low-dose group and the control group werenot significantly different (P＞0.05).Conclusions1. His(10-3～10-6mol/L)could stimulate the cell proliferation in a time-dependentbut not dose-dependent manner and reached the maximal effect at 10-7 mol/L.2. XFDCT good inhibited histamine-induced by the proliferation of ASMC, andthere is a certain time and dose dependent manner.3. XFDCT could treat asthma by inhibiting the proliferation of ASMC. |
PDF Full Text Request