Influenza Virus Infection Of The Molecular Mechanism Of Host Cell Apoptosis Induced By Drosophila Ubiquitin-protein Dhr6 Preliminary Study

Part I: Cell apoptosis is a very important process in the cell's lifespan. It plays important roles in the control of self-stabilization, normal development and carcinogenesis et al. Now, there are many researches indicating that many kinds of host cell apoptosis can be induced by virus infection, such as influenza A virus. But the mechanisms are still unknown. For example, we are still unclear about what kinds of virus proteins induced host cell apoptosis, and what pathways are enrolled in this process and so on. Apoptosis-inducing factor (AIF) and mitochondrial endonuclease G (Endo G) played important roles in the cell apoptosis, but the manners they have in this process are still unclear and there are different manners in different situations. In this thesis, we firstly identified that AIF and Endo G played crucial roles in the nuclear apoptosis induced by influenza A virus infection, and we identified the relationship between AIF/Endo G and caspase activation predominately. We found that there was a difference in the manner of the function between these two molecules. We also checked the relationship between AIF/Endo G and P53 activation and Hsp70 in the process of apoptosis.Part II: Protein ubiqutination is a very important post-translational modification in the regulation of protein function, and we also have a verity of knowledges in the ubiqutination induced protein degradation, but there are many different functions of this modification in different target proteins in different situations. Drosophila Dhr6 is a homolog of Yeast Rad6 protein. Rad6 functions as an E2 enzyme in the protein ubiquitination, and plays important role in the process of DNA damage repair. But there are little publications about the function of drosophila Dhr6. We focused the sub cellular localization of Dhr6 and the localization response of the treatment of DNA damage reagent MMS. We also analyzed the other E2 protein in drosophila, DubcX, and found that these two molecules have different function manners against MMS treatment. At the end of this thesis, I also optimized the experiment conditons of Dhr6-RNAi.