Movement Aortic Endothelial Function In Hypertensive Rats And Its Mechanism

Objective: Hypertension is the main fisk factor for cardiovascular and cerebrovascular disease with high incidence of disease and mortality in China. In recent years, along with unceasingly thoroughly discovered to the primary hypertension pathogenesis research that,the blood vessel endothelial cell and the primary hypertension have the close relation, the main performance for the blood vessel endothelial cell secretion many kinds of active material to the blood vessel tensity adjustment imbalance, the blood vessel structural change, causes the blood pressure to elevate. The normal blood vessel endothelial cell function maintains the blood pressure stable key. NO is synthesized and secreted by the blood vessel endothelial cell,it is important for adjusting blood vessel tensity matter, and a important sign to blood vessel endothelial cell damage. The massive research indicated that, in the blood serum, the NO main origin mainly is produced through eNOS and correlation accessory factor catalysis substrate L-Arg, its content develops the very important function to the hypertension occurrence and regulated by many kinds of factors. At present ,the domestic and foreign research hvve discoved that HSP90 and c-Src are new adjustment to the cardiovascular disease, play the vital role in adjusting eNOS to generate NO. Blood plasma ox-LDL is a eNOS major inhibitor, is important attribute for many cardiovasculars diseases occuring. Suitable exercise could reduce the blood pressure, improves the hypertension patient cardiovascular system function, takes one kind of hypertension the non- pharmacotherapy already widespread applicated in the practice,But its function mechanism whether have related with exercese enhance the HSP90 expression,the c-Src activeness and ox-LDL express, at present not yet sees the detailed report. The research observe training effects on aorta blood vessel endothelial cell HSP90 expression,the c-Src activeness and blood plasma ox-LDL content of spontaneously hypertensive rats(SHR),inquiry into the feasible aerobic sports prevent from the development of hypertensive and cardiac hypertrophy as well as its mechanisms,thus exercise provides the new academic bases for the prevention and cure of the hypertension and the cardiac hypertrophy occurrence and development.Method: The male SHR are randomly divided into the SHR group(S)and training SHR group(T);The male Wistar rats are the normal matched control group(C).T group carries on 90 minutes swimming 6 time a week,all rats feed normal feedstuff for 8 weeks. Determines the rats blood pressure and the body weight each week during experimental period, determines rats aorta blood vessel endothelial cell HSP90 expression,the c-Src activeness, as well as blood serum NO and blood plasma ox-LDL content.Result:1.Blood pressure have no obviously difference before swimming of two SHR group(160.6±4.58mmHg Vs 161.5±9.33mmHg), the blood pressure of Training group notable degrade compares with SHR group (157.6±7.06mmHgVs185.9±6.64mmHg) (P<0.01) after 8 weeks.2.The training group compare with SHR group, the aorta blood vessel endothelial cells HSP90 expression, c-Src activeness and the blood serum NO content all go up obviously(P<0.05,P<0.01 and P<0.05), explained the exercise has the obvious influence to the SHR aorta blood vessel endothelial cells correlation active material.3.The SHR group compare with Wistar group, the aorta blood vessel endothelial cells c-Src activeness has no remarkable difference (P>0.05), the Training group compare with Wistar group, the Training group aorta blood vessel endothelial cells c-Src activeness go up obviously (P<0.01), explained the exercise has vital role to SHR aorta blood vessel endothelial cells c-Src activeness elevates.4..The SHR group compare with Wistar group, the SHR group serum NO obviously lower(P<0.01) and blood plasma ox-LDL obviously higher(P<0.01),explained serum NO and plasma ox-LDL have the vital role to the hypertension occurrence.5.The Training group aorta blood vessel endothelial cells HSP90 expression, c-Src activeness and blood plasma ox-LDL content change have a obviously relevance to NO augment, the correlation coefficient respectively is: Pearson = 0.083,0.805-0.749, all significance is P<0.05. Conclusion:The long-term regular moderate exercise training can inhibit the increase of blood pressure in hypertension,its function mechanism possibly is the long-term regular moderate exercise training to make SHR group aorta blood vessel endothelial cells HSP90 expression increase, the c-Src activeness go up, as well as NO the main inhibitor ox-LDL content degrade, they together promote the NO synthesis and secretion, to maintain and improvement aorta blood vessel bast function, thus to achieve depressurization, therefore has provided the theory basis for the hypertension kinesiotherapy.