The Astringent Variable Model Based On The Miniature Pig Teaching Purposes Pulse

1 ObjectiveBy perform analysis on if minipig can be use in pulse teaching model, Establishment andevaluation of unsmooth pulse teaching model based on mini-pig and tentative explore in madepulse model teaching film, more scientific and systematic in studying pulse teaching model. Bythis way, to spread content of experiment teaching and establishment biology pulse model.2 Methods2.1 Feasible study in pulse teaching model based on minipig. Given pentobarbital sodium toabdominal cavity and intraperiton for anesthesia. After stable anesthesia exposed and fixed fourinternal limbs. To mark pulse locations at armpit artery and femoral artery. Defining the marklocations are guan pulse. Experience TCM doctor located the position of pulse and describe the pulsefeature . With NX-8 multifunctional sphygmograph machine taken normal pressure sphygmogramand the ECGⅡ. Recording the four limbs pressure sphygmogram. To statistic and analysesphygmogram parameter.2.2 Establishment and evaluation of unsmooth pulse teaching model based on mini-pig. Givenpentobarbital sodium to abdominal cavity and intraperiton for anesthesia. Experience TCMdoctor located the position of pulse and describe the pulse feature . With NX-8 multifunctionalsphygmograph machine taken normal pressure sphygmogram and the ECGⅡ. For set up pulsediagnosis model by injected Propranolol and Dextran T-500 in Meridian big ears.Observatedminipig`s general reaction (heart rate, skin color ) and finger`s pulse change. With NX-8multifunctional sphygmograph machine taken the pressure sphygmogram and the ECGⅡsynchronizedly. To statistic and analyse sphygmogram parameter and blood parameter.3 Results3.1 Six minipigs successfully were anesthesia. Produce time is 11±2min,maintain time is47±11min. Heart beat and breath beat light increase after anesthesia.3.2 Minipig`s armpit artery sphygmogram more like human`s cunkou pulse sphygmogram.They all have three upper wave crest. At the same, Minipig`s femoral artery sphygmogram morelike human`s femoral artery sphygmogram. They all have tow upper wave crest. After analysesphygmogram parameter, the sphygmogram did not changed in HB,Sup,Sdown,HE/HBcompared with the same value in human..3.3 Change in unsmooth pulse model3.3.1 General situation The heart beat of these Minipigs have declined and breath rate haveslow down after five minutes modeled. At the same time, observing obviously petechiae andecchymosis at skin, tongue and conjunctiva. Set up pulse model successfully in six mini-pigs. It could be obviously observed that the normal pulse changed to unsmooth pulse by pulse diagnosiswith fingers.3.3.2 Before modeled, minipigs have a nomal pulse frequency of about five beats per breath. Itis faster than human`s. It is even, stable, harmonious and forceful with regular rhythms. Thepulse can be recognized by applying three grades of finger force on the three pulse regions withtouching, searching and pressing. After model, minipigs have a unsmooth pulse frequency ofabout four beats per breath. It has the same frequency with human. It is fine, short and slow,coming and going unsmoothly opposite the smooth pulse. The feeling just as light scrapebamboo with a knife.3.3.3 Sphygmogram feature Amplitude in the delined sphygmogram compared with thevalue before modeled. The speed at figure move slowly upward and downward and have somepause and transition compared with the value before modeled. Usually B wave crest mix togetherwith D wave crest. E have a higher location and F wave were not obvious. All of these accordedwith the feature of unsmooth pulse.3.3.4 Sphygmogram parameter the sphygmogram changed in HB,Sup,Sdown,HE/HB,TAB/TAG,TB/TAG compared with the value before modeled. (Apart of SDown P<0.05, the restare all P<0.01).3.3.5 The remarkable changed inηbw,ηre,ηp,Hct of hemodynamics compared with thevalue before modeled.3.3.6 The remarkable changed in CI, SI, HI, Q-ZI, Co, TPR of hemorheology compared withthe value before modeled.4.ConclusionUsing minipig to set up pulse model successfully found that the model using in teaching hasthe character of convenient, feasible, less-damaged, repeatable and position of pulse even andlong. Set up pulse model successfully in six mini-pigs. It could be obviously observed that thenormal pulse changed to unsmooth pulse by pulse diagnosis with fingers. To perfect objectiveevaluation system in pulse model. And to set up many animal pulse model based on minipig`spulse model can fill in the pulse experimental teaching blanks.