Hccb10124 Antifungal Activity Of A Preliminary Study

In this research work, the strain conserved in HCCB10124 (R&D) was found to be strongly active against a series of fungi,some of which are plant or animal pathogenic , such as Candida albicans, Sarcina lutea, etc. The 16S rDNA sequence analysis combined with traditional identification methods was used to identify the strain. Based on the results, this strain was identified as Streptomyces albireticuli.Tests on culture media and cultural conditions for HCCB10124, showed that carbon source and nitrogen source presented the most significant effect on the production of bioactivity.The optimized medium was composed of glucose 2%, soluble amylum 2%,cottonseed powder 3%,ammonium molybdate 0.04%,calcium carbonate 0.4%. The effects of heat and pH on the stability of the bioactive substance in the fermented broth were studied. The water solubility and ion character of the antibiotics were analysized by pH paper chromatography and thin-layer chromatography. The results showed that the weakly acidic antibiotics were stable to heat and pH.The physiological and biochemical characteristics of the highly activitive antibiotic substances were studied in orde to provide helpful information for the further isolation and purification. A brief process for the primary isolation of the active substance was worked out.In all, Streptomyces albireticuli can produce fairly stable and broads pectrum,strongly active antifungi substance. So it's a hopeful antibiotics producing Streptomyces that its antifungal substance will be value of potential application in agriculture and health.