Chronic Lymphocytic Leukemia Igh Gene Rearrangement And Serum Scd23, Tpo Research

Objective To investigate the rearrangment of immunoglobulin heavy chain (IgH)gene at chromosome 14q32 in chronic lymphocytic leukemia(CLL)and explore the relationship between IgH rearrangement and other prognostic markers,such as other chromosome aberrations,expression of CD38 and ZAP-70,and the status of immunoglobulin heavy chain variable genes (IgVH).Methods LSI IGH Dual Color,Break Apart Rearrangement Probe, SpectrumOrange^TMlabeled sequence-specific DNA probe D13S319 for 13q14, SpectrumOrange^TMlabeled sequence-specific DNA probe p53 and CEP 17 SpectrumGreen^TMlabeled probe for 17p13,CEP12 SpectrumGreen^TMprobe for 12,LSI ATM SpectmmOrange^TMprobe for 11q22,LSI MYB SpectrumAqua^TMprobe for 6q23 and and interphase fluorescence in situ hybridization(FISH)were applied to detect chromosome aberrations in 119 newly diagnosed CLL patients.The expression levels of CD38 and ZAP-70 on CD5⁺CD19⁺CD23⁺ cells were analyzed by four-color flow cytometry (FCM).Multiple polymerase chain reaction(PCR)was applied to detect the status of IgVH in 49 CLL patients. Results Out of the 119 patients,IgH gene rearrangement was found in 17 patients(14.3%)with the rate of lgH rearrangement cells from 10.5%～73%. There were significant correlation between IgH rearrangement and del(17p13), del(11q22)or del(6q23)(P=0.003,P=0.032 and P=0.041,respectively).There were no correlation between IgH rearrangement and del(13q14)or +12 (P=0.959,P=0.089,respectively).Additionally,there was no significant difference between the IgH gene rearrangement and sex,age,lymphocyte count,Binet stages,expressions of CD38 and ZAP-70,or IgVH mutation status.Conclusions The IgH rearrangement is a frequent event in CLL,and is associated with unfavorable prognostic factors.Further investigation of the effect of IgH rearrangement on the pathogenesis and progression of CLL is needed.FISH is a rapid,exact and sensitive technique in analysis of IgH gene rearrangement in CLL. Objective To investigate the serum levels of soluble CD23(sCD23)and thrombopoietin(TPO)in chronic lymphocytic leukemia(CLL)and their correlation with other prognostic factors.Methods The serum levels of sCD23 and TPO of 25 CLL patients were detected by enzyme linked immunosorbent assay(ELISA).Flow cytometry was employed to determine the expression of CD38 and ZAP-70 protein.Results TPO level in CLL patients was significantly higher than that in normal control[(67.22～1881.77)ng/L and(70.29-147.98)ng/L,respectively, P=0.003].sCD23 level in CLL patients was significantly higher than that in normal control[(129.80～405.31)U/ml and(0.65～32.99)U/ml,respectively, P=0.000].TPO level was significantly correlated with Binet stages and CD38 expression.Patients in stage B and C had higher level of TPO than those in stage A[(140.57～457.48)ng/L and(121.92～163.83)ng/L,respectively, P=0.014],while TPO level was higher in patients with higher CD38 expression than in lower CD38 expression[(113.23～199.10)ng/L and(141.34～454.92) ng/L,respectively,P=0.033].No significant correlation of sCD23 and TPO levels with ZAP-70 expression,sex,age,peripheral lymphocyte count or lactate dehydrogenase was observed.