| Coronary heart disease, angina, myocardial infarction is of common diseases, multiple, serious threat to human health, prevention and treatment of its Chinese medicine has a long history and characteristics of Chinese medicine that emotional, restaurants, the result of blood disorders Yat poor , Bizu Xinmaitong bleeding, chest-sluggish efforts Stagnation for Chest Pain and heartache. In recent years the treatment of drug research and development is very active in a variety of new drugs, but from the clinical application of the survey, the overall efficiency was not high. A study of this topic to facilitate the taking, side effects of small, traditional products efficacy of a single defect, can be effective components of the heart, brain and blood vessels simultaneously and rehabilitation of drug.In this paper, shamisen complementary medicine for the treatment of coronary heart disease, high blood lipids, such as angina accompanied by the theme for the preparation of quality standards and preliminary efficacy studies.Preparation of the extraction, compared Ginkgo biloba Boiling water, ethanol back, ethanol-shenlu three extraction methods and choose to return the ethanol extraction, concentration of ethanol, alcohol consumption, extraction time and from the number four Factors by L9 (34) orthogonal experiment, the optimization of the effective parts of Ginkgo biloba - flavonol glycosides constituents to the best of alcohol: 70% ethanol from returning three times and five times the volume of B Alcohol, from one hour in the preparation of purified ginkgo study, carried out the inspection diatomite consumption with a total flavonol by HPLC to determine the most appropriate indicators eluting conditions: get rid of 70 percent concentration, Eluting dosage 60 ml, medicines and silicone ratio of 1: 2. Salvia in the extraction process study, to water Danshensu in the active site for the study of indicators, the amount of water extracted from the time and number three Factors by L9 (34) orthogonal conducted experiments to determine the best conditions for the extraction process: Boiling water extraction three times, each time with water of 10 times, from two hours.In the preparation of studies, inspected the matrix, the type of coolant, drug and matrix and the best ratio of liquid droplets, such as temperature conditions, screening of the emitter in diameter size, Disu, and the drops of coolant from the Temperature, determine the silver and Dan pills of the prescription process: Ginkgo biloba extract and Salvia Decoction of Zexie mixed, concentrated into a dry extract stem joined moderate PEG6000, PEG1000 heating melting, infusion liquid paraffin, mixed Uniform contraction condensed into 1, 000 pills, finished using it, easy to carry.A compound of quality standards studies, including characters, TLC study, the total flavonol glycosides Determination of research, check. Salvia respectively, to control medicine as a reference substance and control, the samples were TLC study, sample Yansui, adequate water, ultrasonic extraction for 10 minutes, centrifugal, from supernatant, and dilute acetic acid and hydrochloric acid 1 ml B vinegar 3 ml, Zhenyao extraction, extract water bath home on Zhenggan, the residue of methanol and 10 ml Shi Rongjie, manufactured goods tested solution to silica gel G as the stationary phase, methanol - acetone - formic acid (25 : 10:4) for the start of conduct, removed, dried and tested for chromatography, in medicine and the control by the appropriate location on the spot in the same color, with the reference substance by the corresponding position , In the same shallow dark red dot, the negative control without interference. Methodological study results showed that: reference substance linear relationship, and recovery was 98.74%, reproducibility, stability, good, and the scope of the inspection, the low limit of -66. 7%, higher limit is +100%, the For the determination of precision and accuracy were better, the pills approved 10 samples to determine identification and content meet the requirement. Proved that: The method is simple accurate, high sensitivity and negative non-interference, can be used for the silver Dan quality control pills.In pharmacodynamics study, the mouse test will be divided into seven groups, through research compound silver Dan pills to normal mice and obese mice weight, liver weight, blood lipid indicators (TC, TG, HDL-C, LDL , AI), Lee's index and the impact on its observation of coronary heart disease, high blood lipid angina with the effectiveness of prevention and treatment to determine the active ingredients, for the extraction of the design and testing for the research, identified the compound Interaction, as cardiovascular disease prevention and treatment drugs, the development of a certain value.In the initial stability in the experimental study of this kind to stay at room temperature for a test, speed up the trial, the basic understanding of the quality characteristics of drugs and changes in laws, to stay at room temperature and speed up the test sample tests showed that samples in the normal temperature conditions and constant temperature and humidity conditions Investigation of six months, the indicators are in line with inspection requirements, stability, good. |
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