Aconitum Toxic Components Of Toxic Drugs On The Myocardial Cell Membrane Receptor Mechanism

Objiective:To research the mechanisms of the venenons constituent of the Aconitum—aconitine,mesaconitine,hypaconitine on the membrane receptor of myocardial cell.Methods:Researching the influence that aconitine mesaconitine and hypaconitine act on the adrenoceptor subtype of the myocardial cell by RBA. Researching the effect that aonitine masaconitine and hypaconitine act on the IP3 and cAMP/cGMP of the myocardial cell by RAA.On the basis of these experiment,it is revealed that effect that aconitine mesaconitine and hypaconitine act on the adrenoceptor subtype and signal transduction pathway of the myocardial cell.Results:Aconitine acting on the myocardial cell produce up-regulation of a1-adrenergic receptors,but has no effect on the affinity of a1-adrenergic receptors. Aconitine has no effect on the affinity and quantity ofβ-adrenergic receptors of the myocardial cell.Mesaconitine and hypaconitine have no influence on the affinity and quantity of a1 andβ-adrenergic receptors.After aconitine mesaconitine and hypaconitien acting on the myocardial cell in different time,the IP3 and the ratio of the cAMP/cGMP of MAT increased than normal group.Conclusion:1.Aconitine has no effect on a1-adrenergic receptors,but no effect on the affinity and quantity ofβ-adrenergic receptors of the myocardial cell.Mesaconitine and hypaconitine have no influence on the affinity and quantity of a1 andβ-adrenergic receptors.2.After aconitine mesaconitine and hypaconitien acting on the myocardial cell,the IP3 and the ratio of the cAMP/cGMP of MAT increased3.The toxic effect of aconitine action on the myocardial cell may have multiple signal transduction pathway.