Veratryl American Ginseng "on The Contrary," Experimental Study Of The Compatibility Relations

Objective:To explore whether there is contrary relation between west ginseng and hellebore.Method:①using the following two methods to prepare west ginseng and hellebore test medicine liquids:traditional water-boiled method and acetic acid-boiled/alcohol-extracted method;②using improved Koushi method to obtain acute toxicity of hellebore,west ginseng and cooperative(1:1) groups;③using spectrophotometry to investigate the impact on mouse serum liver/kidney functions(GPT/BUN);④measuring carotid blood pressure directly combined with biological signal recording system to investigate the impact on blood pressure,cardiogram and respiratory of rats;⑤using semi-solid dextrin method to investigate the impact on gastric emptying and small intestine promotion motion on mice;⑥investigating the impact on vomiting pigeons.Results:①LD₅₀ of said three groups are not obtained by traditional water-boiled method.LD₅₀ of hellebore group is 6.4±1.1g/kg.LD₅₀ of west ginseng/hellebore 1:1 cooperative group is 7.2±1.3g/kg.There is no significant difference(P＞0.05) between them.②GPT/BUN levels can be significantly increased(P＜0.05) in hellebore group and cooperative group.There is no significant difference(P＞0.05) between them.GPT/BUN is not significantly changed(P＞0.05) in west ginseng group.③Pigeons vomit extremely significantly(P＜0.01) in hellebore group and cooperative group.No significant difference(P＞0.05) between them.④Gastric emptying motion and small intestine promotion motion are inhibited extremely significantly(P＜0.01) in hellebore group and cooperative group.No significant difference(P＞0.05) between them.West ginseng has no significant effect(P＞0.05) on gastrointestinal motion.⑤30-90min after administration,SBP, DBP and MP are reduced in hellebore group and cooperative group. Respiratory rate and amplitude are increased in west ginseng group, hellebore group and cooperative group.45-90min after administration,cooperative group can significantly increase heart rate(P＜0.05).60-90min,hellebore group can significantly increase heart rate(P＜0.05).75-90min,west ginseng group can significantly increase heart rate(P＜0.05).No significant impact (P＞0.05) on normal cardiac electrophysiology.Conclusion:①Obvious acute toxicity represents in medicine liquid via acetic acid-boiled/alcohol-extracted method.②there is not obvious difference(P＞0.05) between hellebore group and cooperative group.③There is not significant toxicity on liver/kidney function and gastrointestinal motion by west ginseng. It does not cause vomiting,but increases respiratory rate and heart rate.Each group can increases heart rate.It is indicated that toxicity is not incrased in cooperative group, that is,no contrary relation represented between west ginseng and hellebore.It still needs to explore whether or not the relation of them belongs to Shibafan.