Neuroimmunomodulation Network Incoming Signal And Signal Transduction Model

Neuroimmunology is one of the most developed subjects in life science at the end of the 20th century. Neuro-endocrine-immunological regulation network theory—the fundamental of neuroimmunology has achieved great identification by academic circles and correlative experiments and researches have also demonstrated the existence of function regulation effect between the system of nerve and immune. Based on research of neuroendocrine regulation network, relative investigation has proved that there is a functional regulatory relationship between the nerve system and immune system.The argument in this investigation field was focused on signal afferent pathway of neuro-immune modulation network. In our prophase stuty, we found that the lateral hypothalamic nucleus (LH) and amygdaloid nuclear complex (AA) of the rat hypothalamus are referred to the neuroimmunomodulation. Based on the prophase study, we contrast analyzed ingredient of peripheral blood and cerebrospinal fluid of different immunized stage rats by Mass Spectrometry technique and Two-dimensional SDS electrophoresis to explore the up-delivering signals and afferent pathway involved in neuroimmunoregulation and provide experiment data to elucidate furtherly the anatomic construction of neuroimmunoregulation network. In our study, we use normal humans' IgG as immunizing antigen to prepair immune animal model by tail injection, take serum and cerebrospinal fluid from rats after immunized 0, 2, 4, 6, 8 days. Contrast analyzed ingredient of peripheral serum and cerebrospinal fluid of different immunized stage rats by Surface Enhanced Laser Desorption Ionization-Time Of Flight-Mass Spectrometry ( SELDI-TOF-MS) technique, Two-dimensional electrophoresis and Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS) technique are furtherly applied in analyzing the amino acid sequence of 18KD idio-ingredient and determining signal molecules. The core of the empirical study in this thesis is to identify the passible afferent signal and mechanisms of the afferent signal pathway of neuroimmunomodulation network and to provide experiment evidence for illumination the anatomic construction of the neuroimmunologic network.Relative results show that there are 221 significant different peaks which have correlations with immunological function discovered in serum of immune animals by SELDI-TOF-MS. Among the total of 221 different peaks, 173 peaks are high expressions in experimental group and the others are reverse—increases in control group. But no special different peak which has statistical significance are found in cerebrospinal fluid of immune animals. Also it can't observe corresponding equal increase between the special different peaks tested in peripheral serum and cerebrospinal fluid of immune animals. we compare and analyze the possible afferent signals of the neuroimmunomodulation network in the known neuromediators,neuropeptides,hormones and cytokines with the connection of 76 positive differential expression ingredients with highest peak value at immuned 2 days in rat serums detected by SELDI-TOF-MS to suppose possible afferent signals of the neuroimmunomodulation network. Analysis results on 18KD specific dot of 2-DE by MALDI-TOF-MS report 19 correlated peptide sequences derived from 18 substances, the total of 247 amino acids. However, the correlated results can't satisfy any amino acid sequence arrangement of the known substances.So with the analysis above, we consider that these foregoing results demonstrate that 1) The results of theses research include 221 significant different peaks which have correlations with immunological function discovered in serum of immune animals by SELDI-TOF-MS; there are 76 differential signals with highest peak value expressed at immuned 2 days, 1 differential signal at immuned 4 days, 96 differential signals at immuned 6 days; statistic analysis of differential peak has extremely significant difference, even specificity of some peaks manifests all or none character. All the results have supported the agreement with study on the afferent signal pathway of neuroimmunomodulation network "active immunocytes release relevant functional active signals into blood, then start neuroimmunomodulation work by some way. 2) There is lack of evidence to verify immune modulator could carry out neuro-immune modulation by passing through blood brain barrier directly; 3) Possible afferent signals of the neuroimmunomodulation network such as IL-8,IL-15 and IL-18 supposed with relative factors; 4) IL-1 released by active immunocytes in the blood are not the afferent signal from the immune system to participate in the neuroimmunomodulation; 5) It needs more study on the signal which origins from active immunocytes to trigger the regulatory function of the relevant brain area in neuroimmunomodulation network . The afferent signals may be some unknow subtances.