Like Collagen - The Performance Of The Hyaluronic Acid Vascular Stents, And Biocompatibility Studies

Human-like collagen(HLC) was firstly cross-linked with hyaluronic acid(HA) in different ratio by genipin for constructing tissue engineering blood vessel scaffolds.The concentrations of hyaluronic acid in the mixture were 0,0.01%,0.05%and 0.1%.The blood vessel tubular grafts were then fabricated by freeze-drying,finally their physical properities and biocompatibility were studied.1.Microstructure of different vascular scaffold groups were studied by scanning electron microscope(SEM),The results showed that HLC-HA(0.05%) tubular scaffold exhibited well-distributed interconnecting porous structure and the porosity was 94.38%.2.Element composite of different vascular scaffold groups were studied by X-ray photoelectron spectroscopy(XPS),The results showed that the content of carbon and nitrogen of HLC-HA were between pure HLC's and HA's,but the content of oxygen of HLC-HA was the least of HLC-HA,HLC and HA.3.Tensile test and burst pressure experiment of HLC-HA(0.05%) were performed and the desirable mechanical property with stress of(1000.8±7.9) kPa and burst pressure of (1058.6±8.2) kPa were achieved4.The degradation of HLC-HA(0.05%) blood vessel scaffolds were determined by the degradation experiment in vitro,which can indicate the degradation property of blood vessel scaffolds in vivo.5.Cytotoxicity experiment showed that cytotoxicity grade of HLC-HA(0.05%) scaffolds was first grade and non-cytotoxicity.6.The cytological experiments indicated that endothelial cell could grow,proliferate and adherent on the surface of HLC-HA(0.05%) vessel scaffolds much better than the HLC control,and the cell morphology was normal which showed that the cell compatibility of HLC-HA composite is good;7.Hypodermic embedding experiments of mice exhibited that HLC-HA(0.05%) scaffolds had favourable cytocompatibility.The subcutaneous tissue was analyzed by HE staining and transmission electron microscopy 1 weeks,3 weeks,5 weeks and 9 weeks later, respectively.The results indicated that HLC-HA(0.05%) had favourable hisocompatibility and biodegradability.