Astragalus Polysaccharides On The Human Heart Microvascular Endothelial Cells In Ischemia Reperfusion Injury Impact Study

ObjectiveMyocardial ischemia-reperfusion injury is a problem during the treatment of reperfusion in acute myocardial infarction of coronary heart disease.Animal experimental studies have found that astragalus polysaccharides(APS) has a good influence in counteracting myocardial ischemia-reperfusion injury.So in this experiment,we mainly study the influence of APS on HCMEC of myocardial ischemia-reperfusion injury,and discuss the influence of APS in counteracting myocardial ischemia-reperfusion injury much more pertinently.Methods1 The culture of human cardiac microvascular endothelial cells(HCMEC): Establish a stable cell experiment platform after the recovery,training, passages of cardiac microvascular endothelial cells.2 The cell proliferous experiment of APS on normal cultured HCMEC:Detect the cell activity changes by MTT method.Observe the cell proliferation of normal cultured HCMEC that are treated with different concentrations and different times.3 The effect of APS on HCMEC after ischemia-reperfusion injury:Detect the cell activity changes by MTT method that is dealt with different concentrations of APS.4 The effect of APS on protein expression of P-selectin and E-selectin:Observe the protein expression of P-selectin and E-selectin with different concentrations of APS during the early time of ischemia-reperfusion injury by cell elisa.Results1 The HCMEC bodies were pachynsis and transparent and showed in rhombus and polygon shapes,cell nuclears were small and round after recovered.HCMEC was monolayer growth and showed a cobblestone appearance.2 The proliferation of HCMEC was higher compared to the control group when the concentration of APS was in 151.25μg/mL～18.78μg/mL.During these experiments,the proliferations of HCMEC were not distinct in 18.78μg/mL and 151.25μg/mL,however,they were notable in 37.56μg/mL and 75.13μg/mL.There was no evident change in the proliferation of HCMEC when treated separately with 25,50,100μg/mL of APS for 4h,6h,8h.3 The cell activity decreased significantly in ischemia-reperfusion injury compared to normal group.However,the APS could increase the cell activity.4 The protein expression of P-selectin and E-selectin increased significantly in ischemia-reperfusion injury compared to the normal group,however,the middle and high doses of APS could inhibit the expression significantly except the low dose.Conclusion1 In a certain range of dosage,APS could promote the proliferation of normal cultured HCMEC,but it needs longer time to take action,maybe at least 8 hours.2 In a certain range of dosage,APS could protect HCMEC in ischemia-reperfusion injury.3 In a certain range of dosage,APS could inhibit the protein expression of P-selectin and E-selectin during the early time of ischemia-reperfusion injury.