Glp-1 Protective Effect On Stz-induced Model Of Ad

Propose:To investigate GLP-1 's protection function to AD animal model induced by STZ.The study aim is:(1)effect of GLP-1 on behavior of rats treated by STZ;(2) effect of GLP-1 on Aβin serum and brain of rats treaded by STZ.Method:Male Wister rats were fed for a week and the weaker ones,which have a bad appetite or lost weight quickly,were excluded.Then 30 qualified rats were left after the choice of Morris water maze,through which the Down's syndromes(the average of incubation period is over 50s) and the ones which didn't swim well were excluded.The 30 qualified rats were divided into three groups:black comparison group,AD model group and GLP-1 protected group.There are 10 rats in each group respectively.The rats in AD model group were injected 10%STZ through head,and GLP-1 group 10%STZ and GLP-1,while the black comparison group only Physiological Saline.The wound was managed treated with antibiotic.7 days,14 days and 21 days respectively after the operation,vein blood of the rats was draw and the level of serum was tested by unsaturated radioimmunoassay.The behavior of the rats before and 21 days after the Morris water maze test were compared.After putting the rats to death,the changes of Aβin their brain organization were tested.Results:(1) The avoiding latent period of rats treated by STZ was delayed as compared with control rats.GLP-1 reversed the damage of STZ to shorten latent period of rats compared with the rats treated by STZ.(2) The rats of control swam more in the area where the platform had installed compared with the rats treated by STZ.The rats treated by GLP-1 swam more in the area where the platform had installed compared with the rats treated by STZ.(3) Serum Aβof rats treated by STZ was obviously elevated as compared with control rats and the rats treated by GLP-1.(4) More immune staining neurons were seen in the brain of rats treated by STZ as compared with control rats and the rats treated by GLP-1.Conclusion:GLP-1 improved bahavior damaged by STZ,decreaded serum Aβinduced by STZ and weaken Aβexpression in brain induced by STZ.It is possible for GLP-1 to improve AD-like behavior and pathology.