The Identification Of A Marine Fungi And Their Secondary Metabolites

Ocean is the place of origin of life, the biological diversity of which is far more than terrestrial organisms. There are about 100 million kinds of marine microorganisms as much. The species diversity of marine microorganisms determines the diversity of its metabolites, which micro-organisms living under the marine environment is a new important source of biologically active substances. The synthesis of marine microorganisms is excellent, which can produce much bio-active substance, the structures and functions of which are very specific. Domestic and foreign scholars have isolated a large number of active substances from marine bacteria, actinomycetes and fungi, some have unusual structures, some have the unique activity, and some combination.In this paper, strain 0312F1, the anti-TMV, anti-tumor activity of fermentation broth of which was screening to be higher than other marine fungi was selected to be tested. By morphology and molecular identification methods, is identified as Penicillium oxalicum. After growing on the rice culture, the fermentation of 0312F1 was extracted by methanol, the anti-TMV and anti-tumor activity of which was tested then. The results showed that the active fraction named 0312F1 was water-soluble.Using normal-phase column chromatography, reversed-phase column chromato- graphy and liquid chromatography method, 5 compounds(L-22,L-26,L-28,L-31,L-32)was isolated and identified. The inhibitory effect of compound L-31 against TMV replication was significant, with inhibition rate 72.17% at the tested concentration of 200μg/mL, EC50 value 100.80μg/mL.