| 1 Objective:By observing the thermal adaptation and energy metabolism in rats taking Vc related indicators on the impact of heat adaptation, Vc energy metabolism in rats and its mechanism in order to enhance sport athlete in high temperature environment, ability to provide a theoretical basis.2 Methods8-week-old Wistar rats were 108, weight 200±15g, were randomly divided into 4 groups:A (normal control group), B (exercise group), C (exercise+heat acclimation group), D (exercise+heat adaptation+Vc group); B, C, D rats were generally treadmill training for 5 weeks,6 weeks on the C, D groups heat adaptation (38℃) trained and fed to the D group Vc, increasing exercise training hours per week:1 week of training time as 10min, speed 10m/min; weekly time increments after 10min, the speed increase 3m; the first week of training time for the 4,5,6 40min, speed of 21m/min. By resting state, fixed load condition, state of exhaustion, immediately after exercise were killed, skeletal muscle, serum, measured quadriceps, gastrocnemius muscle tissue Na+-K+ATPase, CK, LD, LDH, SDH activity. SPSS 10.0 statistical software with all the data analysis, the significant level of p<0.05.3 results3.1 resting state Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.01); movement adaptation+Vc+heat group was significantly higher than the other 3 groups (P<0.01); serum tissue in CK activity, motion and heat adaptation was significantly higher (P<0.05); movement adaptation+Vc+heat group was significantly higher (P<0.05); serum levels within the organization LD, LDH activity was not significantly different (P> 0.05); SDH activity exercise+heat adaptation was significantly higher; movement adaptation+ Vc+heat group was significantly higher than the other 3 groups (P<0.01).3.2 Quantitative exercise 1 hour Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.01); movement adaptation+ Vc+heat group was significantly higher than the other 3 groups (P<0.01); serum tissue CK activity in the exercise group, exercise+heat adaptation was significantly lower than the control group (p<0.05). Serum levels within the organization LD, LDH activity were not significantly different; (P> 0.05); SDH content exercise+heat adaptation was significantly higher (p<0.05); Games+hot+VC was significantly higher and the movement group (P<0.01).3.3 immediately after exhaustive Within the skeletal muscle Na+-K+ATPase activity, exercise+heat adaptation was significantly higher (P<0.05); movement adaptation+Vc+heat group was significantly higher and the exercise group (P <0.01); Sports+heat adaptation+Vc was significantly higher than the exercise+ heat acclimation group (P<0.05). Serum CK activity within the organization, sports group, motion and heat adaptation, motion and heat adaptation+Vc group was significantly lower than the control group (p<0.01). Tissue content of serum LD, LDH activity, no significant difference; (P> 0.05); SDH activity exercise+heat adaptation was significantly higher (p<0.05); Games+hot+VC was significantly higher (P<0.01).4 Conclusion4.1 Thermal adaptation and 1 week and Vc can significantly improve the heat stress in rats Na+-K+-ATPase activity, in particular, thermal adaptation and Vc combined effect may be significantly increased Na+-K+-ATPase, SDH activity, indicating thermal adaptation 1 week and Vc combined effects of heat stress to improve aerobic capacity in rats with highly significant results.4.2 at rest, heat 1 week to adapt to the heat stress in rats significantly increased serum CK activity in the quantitative load and CK activity increased after exhaustive state is not obvious, indicating the thermal environment of the body can cause damage, but heat adaptation week can reduce this damage.4.3 Vc is a strong reducing agent has a clear role of free radicals protecting the body, but can not defense the body under high temperature damage. |
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