Happy Bulk Compound Intestinal Absorption Study

Kaixinsan is a kind of elementary prescription to treat the traditional medicine emotion-thought disease. It is consisted of radix ginseng, poria, radix polygalae and Rhizoma Acori tatarinowii. It has been recorded in the old medical books, which is mainly to treat emotion-thought disease of traditional Chinese medicine and especially psychosis in clinic, which is familiar to the depression disease of western medicine.Using an improved rat everted gut sac model, we investigated the intestinal absorption effects of kaixinsan radix ginseng, poria and polygala tenuifolia in rats, furthermore by HPLC-MS, and we detected the intestinal absorption effects of kaixinsan, especially polygala tenuifolia, radix ginseng. Using GC-MS, we detected the main absorbed compound in volatile oil from R. Acori. It provided the evidence in the progress of the basic material effect of Kaixinsan.Using an improved rat everted gut sac model and using the HPLC-ESI-MS to detect the absorption of Kaixinsan. We determined the main compositions to absorption in kaixinsan were the compositions in radix ginseng and radix polygalae. The major compositions to absorption in radix ginseng are the ginsenoside and the major compositions to absorption in radix polygalae are the polygala tenuifolia Willd. Combined the information of the MS-MS and UV, and inquired about the DNP2003, we can assessmented initially the main compositions were ginsenoside Rb1,Rb2,Rg1,Rg2,Rc,Rf,Ro,N-R2 etc and Feruloyl sucrose, Sinapoyl sucrose, Trimethoxy cinnamoyl sucrose, Bis-Sinapoyl sucrose,p-Hydroxy benzoyl trimethoxy cinnamoyl sucrose and Sinapoyl trimethoxy cinnamoyl sucrose etc.Using an improved rat everted gut sac model and using the GC-MS to detect the absorption of Rhizoma Acori tatarinowii. By retrieving the GC-MS database and verifed the relative information of the MS, We determined the main compositions to absorption in Rhizoma Acori tatarinowii are the six feature compositions which were named methyl eugenol, cis-methyl isoeugenol, trans-methyl isoeugenol, a-asarone, P-asarone and y-asarone. In this foundation, In vivo single-pass perfusion model was used and the concentrations of a-Asarone in the perfusate were determined by HPLC. All the perfusate solutions collected were weighed and gravimetric method was utilized for the calculation of absorption kinetics parameters. a-Asarone could be absorbed in general intestinal segments, and the absorption of a-Asarone in the intestine is a first-order process through passive diffusion mechanism.By the modern pharmaco-theory, ginsenoside are the main effective compositions to cure the disease of central nervous system and have two-ways regulation effect. According to the different of dosage and body's functional status, ginsenoside can enhanced the effect to central nervous system. In this foundation, improved rat everted gut sac model and HPLC were used to investigated the absorption of ginsenoside Rgl and ginsenoside Rbl, the result indicated that ginsenoside Rgl and ginsenoside Rbl have good absorption in intestine.Rhizoma Acori tatarinowii was a traditional drug of causing resuscitation by administering aromatic drugs. It is wildly compatibility with other traditional Chinese drugs cured the disease of the cerebral disease. It increased the permeability of the blood-brain barrier. So we had consideration for add the volatile oil from R. Acori in ginsenoside Rgl and ginsenoside Rbl to infer the effect of the absorption in intestine. In this foundation, improved rat everted gut sac model and HPLC were used, and the result indicated that the volatile oil from R. Acori can inhibited the absorption of ginsenoside Rgl and ginsenoside Rb1 evidently.