The H1n1 Mouse Model And Radix Particles Of The H1n1 Mouse Model Of The Role Of Research

Influenza A H1N1 flu first discovered in March 2009 and quickly spread around the globe. At first World Health Organization (WHO) called this initial influenza the "swine influenza", later it was called "H1N1 influenza". June 11, WHO announced that influenza H1N1 pandemic warning level upgraded to 6. The pathogens of this H1N1 influenza is a new strains of influenza virus. This novel contail three gene fragments: swine flu, bird flu and human influenza viruss. Oseltamivir have a good treatment for the new novel influenza. However, in many developed countries, many patients have had drug resistance to Oseltamivir. It is essitial to establish animal model and to provide an important basis to study the pathogenic characteristics of the virus, prevention and control technology, pathogenesis, drug screening, evaluation and other aspects of vaccine research studies when we faced such a complex situation. Homogeneous mouse genetic background, individual differences is small, accurate and reliable test results.We compared A/California/4/2009 and A/California/7/2009 that popular in 2009. It can be found that A/California/7/2009 had higher infection replication than A/California/4/2009 from the experimental results in vivo and in vitro. So we chose A/California/7/2009 to establishs an animal model.There are sialic acid a-2,3 sialic acid receptor and galactosidase a-2,6 galactosidase receptor in mouse trachea and lung tissue, and influenza H1N1 influenza virus have easy integration to these two receptors. Genetic background of mice is homogeneous, having little difference and accurate and reliable test results. We used BALB/c mouse as an animal model. We can confirm that from the first part of the experiment. A/California/7/2009 can infect BALB/c mice directly, not need passaged for many times to adapt in mouse lungs. We infected BALB/c mice through the intranasal. Mice showed weight loss, vertical hair and low spirit 3 days after infection. Mice began to die 4 days after infection. There are 100%mortality within 8 days after infection. Mice were sacrificed. The results of HE staining showed that pulmonary septum widened and inflammatory cells infiltrated, blood vessel in pulmonary interstitium dilatated and congested, edema fluid and inflammatory cells exuded into alveolar space, and the necrotic mucous membrane of the bronchiole shed. Severe diffused interstitial pneumonia was the main manifestation. Influenza A virus can be detected in many organs.Through the text of molecular biology and pathology, CA7 virus can be detected in more organizations of the body. The main lesion of mice lung tissue was severe diffuse interstitial pneumonia. These datas proved that animal models of BALB/c mice infected with influenza A H1N1 virus were established. This study establish an evaluation platform for the effective anti-influenza drugs and vaccines to prevent influenza A H1N1 influenza virus.On the basis of articles reported, many chinese medicine have a therapeutic effect on seasonal influenza in the anti-virus, enhance immunity, elimination of inflammation. In the past Influenza, Banlangen have a better effect in prevention and treatment. However, the role in the treatment of influenza A H1N1 is not clear.In this study, we choose Banlangen and use mice infected by Influenza A H1N1 influenza as a model for drug evaluation, study the effect against A H1N1 influenza virus in both anti-virus and eliminate inflammation. The results showed that Banlangen can prolong the survival days of mice infected by influenza A H1N1 and improve survival. After dissecting mice, we can see lung lesions of mice in Banlangen group smaller than mice in model group, so Banlangen can ease the lung lesions due to influenza A H1N1 virus.The result indicated that Banlangen has a protective effect in mice infected by A H1N1 virus. But there are no significant differencethe in viral loads in lung tissue in model group and Banlangen group.This showed that the effect against A H1N1 virus is not obvious. It may play the role of anti-influenza through regulating cellular immunity.The mechanism of its anti-virus is in further experiments.