Timp-1 Expression In Human Periodontal Ligament Cell Proliferation And Alp Activity

[Objective]:This series of experiments aimed at evaluatingthe biological effects of tissue inhibitor of metalloproteinase-1(TIMP-1)on the proliferation and alkaline phosphatase(ALP) activity in human periodontal ligament cells(HPDLC). Choicing optimum time and concentration, can provide experimental basis for effective promoting the proliferation of PDLF in vitro.[Method]:We cultured HPDLC from the third generation in vitro. The cells of 5 to 7 generation were divided into a control group and four experimental groups. Dubecco's modified eagle medium(DMEM) containing 10%FBS was in control group, the cells of experimental groups were treated with four different concentrations(1ng/ml,10ng/ml,50ng/ml,100ng/ml) of TIMP-1. Thiazoly blue (MTT) colorimetric assay and ALP activity analysis were used at different time points (the 24th,48th and 72th hours) to determine the proliferation and ALP activity of HPDLC after different doses of TIMP-1 were added into the culture medium.[Result]:(1)cell proliferation:When compared with control group, lng/ml TIMP-1 at 24th,48th and 72th hour, lOng/ml TIMP-1 at 48th and 72th hour, more significantly promoted the proliferation of HPDLC.(2) ALP activity:lng/ml TIMP-1 at 24th,48th and 72th hour, lOng/ml TIMP-1 at 48th hour more significantly increased the ALP activity of HPDLC.[Conclusion]:This study has indicated that TIMP-1 may simultaneously promote the proliferation and differentiation of human periodontal ligament cells. This conclusion indicated that TIMP-1 has up-regulation effect for HPDLC proliferation.This study may provide a experimental basis for new attachment and remodelof periodontium after periodontitis treatment.