Neisseria Gonorrhoeae Ir Area Base Deletion And Its Mdr Research

Objective 1-To observe the resistance frequency and trends of 91 Neisseria gonorrhoeae(N.G.) strains to five antibiotic agents in Shanxi.2.To study the relationship between mutation of the 13bp inverted repeat sequence in the mtrR promoter region and multiple resistance of Neisseria Gonorrhoeae (NG) in Yuncheng,Shanxi.Methods 1.The disk diffusion method was used to test the susceptibility to 5 antibiotic angents including penicillin, tetracycline, ciprofloxacin, ceftriaxone and spectinomycin.2.The producing ofβ-lactamase was determined by nitrocefin.3.The multiple transferable resistant system's (mtr) inverted repeat sequence (I R) of Neisseria gonorrhoeae was sequenced and analysed after amplification by polymerase chain reaction (PCR).Results 1.The resistance rates of 91 strains to penicillin, tetracycline, ciprofloxacin, ceftriaxon,spectinomycin were respecticely 69.23%,87.91%,97.80%,0,5.49% in Shanxi;penicillinase-producing Neisseria gonorrhoeae(PPNG) accounted for 41.76%(38);resistance rates of multiple-resistance isolates to 5 antibiotics were 94.51%(86).2.Positive rates of multiple-resistance isolates to 5 antibiotics were 85.71%(30),19 multiple antibiotic resistant strains had one single base deletion (T/A) in IR gene in Yuncheng.Conclusions 1.Penicillin, tetracycline, ciprofloxacin cannot be used as routine agents to treat gonorrhea;ceftriaxone and spectinomycin should be used as the first line agents in treating gonorrhea at present in shanxi area,however attention should be paid to the appearance of some resistance strains to spectinomycin.2.Multi-drug resistance is widespread in Shanxi 3.The single-base deletion of the 13bp inverted repeat sequence in the mtrR promoter region of neisseria gonorrhoeae may enhance the resistance of neisseria gonorrhoeae to antibiotics.