The. Ruboxistaurin Mouse Oxygen-induced Retinopathy Retinal Vegf Expression

Objective:To establish the animal model of Oxygen-Induced Retinopathy of prematurity(OIR),The models were given Ruboxistaurin by gastric perfusion after OIR model inducing success. Examining the expression of VEGF in the retinal tissue of the models.Methods:To establish the animal model of the Oxygen-Induced retinopathy of prematurity。One hundred and twenty 7-day-old C57BL/6J mice were randomly divided into four groups:they are normal group, hyperxia group, Ruboxistaurin treatment group and control group。All mice except the normal group were exposed to 75% oxygen for 5 days and then exposed to room air 5 days to establish OIR models. The last two groups, Ruboxistaurin treatment group and the control group;were further devided into three groups that include little dose group,middle dose group and large dose group.The expression of VEGF protein was assessed by immunocytochemistry assay, and the VEGFmRNA was studied by reverse transcriptase PCR(RT-PCR)。Results:The results of immunocytochemistry assay show that VEGF was detected in normal group, and its expression increased in hyperxia group (P<0.01)。The changes of VEGFmRNA paralleled to the changes of its protein.The expression of VEGF in the three treatment groups were 132.7713±5.5100,138.0096±1.8700,143.2910±4.6600, and the VEGFmRNA were 0.8973±0.0600,0.8254±0.0400,0.6183±0.0300, Compared to the hyperxia group, VEGF expression in Ruboxistaurin treatment group were decreased The expression of VEGF in normal group,hyperxia group, Ruboxistaurin treatment group present significant difference (P<0.05).The expression between hyperxia group and control group has no difference. (P>0.05)Conclusion:The expression of VEGF were increased in Oxygen-Induced Retinopathy of prematurity.PKC activation is an essential component of the signal transduction pathway to increase the VEGF expression in OIR. Ruboxistaurin could down-regulate the expression of VEGF in the models.