.5 - Aza-2'-deoxycytidine On Human Cervical Cancer Cell Lines Hela And Siha Cells, Syk Gene Aberrant Methylation And Expression Of The Impact

Objective To investigate the effect of different concentrations of demethylating agent 5-Aza-2',-deoxycytidine (5-Aza-CdR) on the expression and its methylation of Syk gene promoter region in cervical cancer cell lines of HeLa and SiHa.Methods Methylation-specific PCR (MSP) was utilized to detect the methylation state of Syk gene promoter in cervical cancer cell lines of Hela and SiHa; Activation in HeLa cells proliferation was monitored by MTT assay pre/post treatment at different concentrations of 5-Aza-2'-deoxycytidine; MSP was also applied to detect the changes of methylation on Syk gene promoter in cervical cell lines of HeLa and SiHa pre/post treatment with different concentrations of 5-Aza-2'-deoxycytidine; in the meantime, the expression level of Syk mRNA was detected using RT-PCR pre/post treatment with 5-Aza-CdR.Results The methylation of Syk gene promoter was found in HeLa cells and which was not present in SiHa cells;the proliferation of Hela cells could be inhibited by 5-Aza-2'-deoxycytidine, its optimal concentration to inhibit the proliferation of HeLa cells was 1μM. It was also shown that treatment with 5-Aza-CdR could induce the demethylation of the Syk gene promoter, its optimal concentration was luM; further investigation found that the demethylation with 5-Aza-CdR restored Syk gene expression in HeLa cells.Conclusion It was found there was methylation in the Syk gene promoter in Hela cells, its methhylation could be demethylated by 5-Aza-2'-deoxycytidine, which resulted in restored the expression of Syk gene.5-Aza-2'-deoxycytidine could inhibit the proliferation of Hela cells.