Cork Concocted Principles And Quality Standards

Purpose:Cortex Phellodendri Chinensis is the dry bark of Phellodendron chinense Schneid.,which was commonly used to purge fire for removing toxin and eliminiate heat for drying moistenning. Cortex Phellodendri Chinensis with saltwater ,which is always applied to nourish Shen Yin in clinic ,is the major and popular processed product now,But its detail processing technology is not very clear.So the purpose of this study is to optimize the processing technology,research the changes of constituents,compare the function of nourishing Shen Yin before and after processing,establish the quality standard of Cortex Phellodendri Chinense and Cortex Phellodendron Chinensis with salt water,and clarify the principle of Cortex Phellodendri Chinensis processed with saltwater.Material:Cortex Phellodendri Chinensis,Berberine hydrochloride,Berberrubine hydrochloride,Phellodendrine hydrochlorideMethod:1.The content variation of Berberine hydrochloride and Berberrubine hydrochloride were selected as quality index,the influencing factors such as processing technology of processing temperature,processing time,the weight of salt and soaking time were optimized by L9(34) orthogonal design experiment.Based on this,industrial technology with salt water was determined according to quality of 10 piece of processed products.2. Compare the constituents between the crude and the processed product by TLC and HPLC.3. By intragastricing the rat mould of"hyperthyreosis"and Shen Yin deficiency symptoms"with the crude samples and the processed samples separately for three weeks,the content of FT3 ,FT4 in blood serum,17-OHCS in urine,heart rate and the activity of Na+-K+-ATPase were determined in order to compare the function of nourishing Yin before and after processing. The effect of the Cortex Phellodendri Chinensis and its processing product on the high temperature of mouse caused by yeast was test.4.Serving berberine hydrochloride,berberrubine hydrochloride and phellodendrine hydrochloride as index, the quality criteria of Cortex Phellodendri Chinensis and its processed product was established. The impurity,moisture content,ash content and extractive of the Cortex Phellodendri Chinensis and its processed product of ten habitats were determined.Result:1.Through single factor experiment and L9(34) orthogonal design,the optimum processing technology was set as follow:per 100 kg Cortex Phellodendri Chinensis used the salt 2 kg,solved by 30% of water of the medicinal materials.soaking 1 hour,frying 8 min at the temperature of 150～160℃;and industrial technology was set as follow: per 100 kg Cortex Phellodendri Chinensis used the salt 2 kg,solved by 15% of water of the medicinal materials.soaking 1 hour,frying 5 min at the temperature of 150～160℃.2.Part of berberine hydrochloride became berberrubine hydrochloride in processed products,and the the content of berberrubine hydrochloride increased at the beginning,the peak was 1.86% at the condition of 180℃and 60min,at last it went down.The study also showed that the content of Phellodendrine hydrochloride both in the product with saltwater and in the dried product was more than that of the crude product.3.The crude Cortex Phellodendri Chinensis and the processed products could reduce the content of FT3, FT4 in serum ,decrease heart rate and the activity of Na+-K+-ATPase,and high dose of the processed product could lower 17-hydroxy corticosteroid in urine significantly.both of them had the effect of nourish Shen Yin,and the product with salt water was powerful than the crude product.The low dose of the crude Cortex Phellodendri Chinensis could inhibited significantly the augmentation of mouse'temperature,and the processed worked insignificantly.4.The item in the methodology inspection of berberine hydrochloride,berberrubine hydrochloride,phellodendrine hydrochloride were qualified. The content of of berberine hydrochloride and phellodendrine hydrochloride in the Cortex Phellodendri Chinensis was tentatively fixed more than 3.00% and 0.41% respectively;The content of berberine hydrochloride,berberrubine hydrochloride and phellodendrine hydrochloride in the produnct with saltwater was more than 3.00%,0.08% and 0.38% respectively. Conclusion:1.The optimum processing technology of Cortex Phellodendri Chinensis with saltwater carried out in the amplification pilot,and was proved stable and feasible.2.Some qualitative and quantitative changes had taken placed in a group of constituents of alkaloids in Cortex Phellodendri Chinensis after processing.The changes was important to process theory of Cortex Phellodendri Chinensis with saltwater.3. Cortex Phellodendri Chinensis and the processed product with saltwater had function of nourish Shen Yin,and the processed product with saltwater was stranger than the crude product,which proved that the process with saltwater could bring drug into Shen Jing,and Strengthen the efficacy of nourish Shen Yin.4. Cortex Phellodendri Chinensis and the processed product with saltwater had function of eliminiating interior heat,and the crude product was better than the processed product with saltwater,which proved that the process with saltwater could moderate the coldness of Cortex Phellodendri Chinensis,and suggested that the crude product could be proper to apply in eliminiating interior heat.5. The impurity,moisture content,ash content and extractive of Cortex Phellodendri Chinensis and the processed example with saltwater of ten habitats were determined and the quality criteria of Cortex Phellodendri Chinensis and its processed product was stable and feasible,which could be used to evaluate and control their quality.