Tippy Mushroom Fruiting Body Polysaccharides Extracted </ Sub> H <sub> 22 Tumor-bearing Mice Antitumor Effect

Objective:To optimize Mao jian mushroom sporocarp polysaccharide extract, discusses the optimum methods of extracting Mao jian mushroom polysaccharide. Mao jian mushroom polysaccharide of different concentration acts on H₂₂ tumor-bearing mice, through detecting expression of apoptosis related gene Caspase-3,Caspase-8 mRNA ,analyse the molecular mechanism of apoptosis induction, indices of thymus and spleen,inhibitory rate of H₂₂ tumor are observed after different concentration Mao jian mushroom polysaccharide acting on H₂₂ Tumor-bearing mice. To provide new ideas and new methods for treating liver cancer on the clinical medicine.Methods: Using hot water to extract the Mao jian mushroom polysaccharide, compare extraction efficiency of Mao jian mushroom polysaccharide at different temperature. Mao jian mushroom polysaccharide of different concentration acts on H₂₂ tumor-bearing mice , using the formula: thymic index (mg/ g) = 1000×thymus weight (g) / body weight (g), spleen index (mg/ g) = 1000×splenic weight (g) / body weight (g) , inhibitory rate of tumor =﹝1﹣tumor weight of drug group(g) / tumor weight of negtive group(g)﹞×100% to calculat the thymus and spleen index and inhibitory rate of tumor. To observe the change of the H₂₂ Tumor-bearing mice immune system and the influence of tumor growth.Using the HE method to observe the morphological changes of apoptotsis, Using the RT-PCR technology to analysis expression of caspase-3 and caspase-8 mRNA.Results: In the process of Mao jian mushroom polysaccharide extract,when the temperature is 80℃,90℃and 100℃, and rinsing extract, extraction efficiency of Mao jian mushroom polysaccharide respectively is 3.712%,2.794%,2.040%;When the temperature is 80℃and 90℃,and not rinsing extract, extraction efficiency of Mao jian mushroom polysaccharide respectively is 1.530%,0.965%. polysaccharide group compared to blank group and negative group(p>0.05), spleen index is not statistically significant, but the thymus index increases obviously. polysaccharide group and positive group compared to negitive group , the weight of tumors is decreased obviously . HE showes the obviously morphological changes of tumor celles in high,medium,low concentration polysaccharide and negative groups, and tumor celles appear large necrotic . The measured result of RT-PCR technology indicates that caspase-3, caspase-8 mRNA are expressed in high,medium,low concentration polysaccharide groups,but they are lower than the negative group(p<0.05).In addition, the expression of caspase-3,caspase-8 mRNA exist high,medium and low differences in high,medium,low concentration polysaccharide gtoups(p<0.05).Conclusion: the optimal temperature of Mao jian mushroom sporocarp polysaccharide extract is 80℃;extraction efficiency of Mao jian mushroom polysaccharide respectively is higher after rinsing extract; Mao jian mushroom polysaccharide can enhance immune function of mice and suppress tumor growth;Mao jian mushroom sporocarp polysaccharide can promote H₂₂ cell apoptosis ; caspase-3 and caspase-8mRNA are all expressed(p<0.01),so mao jian polysaccharide can promot apoptosis by means of death receptors.