Systematic Analysis Of The Conservation In Heat Shock Proteins And HSP70

Heat Shock Proteins (HSPs) are a class of Stress proteins which existence are very widespread range from bacterial to humans. HSPs are normally classified into six families according to their molecular weight: HSP100, HSP90, HSP70, HSP 60, HSP4 and Small HSP. Why are HSPs so important? When excessive heat or other stress are applied to organisms, HSPs will dramatically upregulate and protect organisms. Although HSPs play such an important role, the numbers of known HSPs are very rare, especially in species other than human. To investigate the origin and evolutional conservative of HSPs and is there possible to find out many new genes, we attempted to determine near-complete HSPs repertoires by searching whole genome sequences of 8 species, including Fruitfly, Ciona Savignvi, Zebrafish, Xenopus Tropicalis, Chicken, Rat, Mouse and Chimpanzee. To make our conclusion reliable, we systematically analysis 52 amino acid sequences of HSP70 from 9 species, including Fruitfly, Ciona savignvi, Zebrafish, Xenopus, Chicken, Rat, Mouse, Chimpanzee and Human.The main results are as following:1. The results of sequences alignment showed that apart from Ciona savignvi of Small HSP, all other species have a large number of potential functional near-complete heat shock proteins.2. The results of sequences alignment showed that HSPs are high conserved on evolution.3. The Neighbor-Joining Phylogeny tree of HSP70 showed that heat shock proteins could hardly clustered according to species which means HSPs are highly conserved on evolution.4. To make our results more reliable, we predicted and removed the signal peptides from 52 amino acid sequences by SignalP3.0. Then we used MEME4.5.0 to analysis conserved motifs of these sequences. Our result showed that HSPs are highly conserved again,In conclusion, our results showed heat shock proteins are highly conserved and hardly have any mutation. There are a large number of new potential functional HSPs are not discovered recently. Our results may provide important clue for further studies on the conservative of HSPs and finding new genes.