Purification And Characterization Of Cellulase From Hindgut Of Termite

To screening a high yield and high efficiency of cellulolytic cellulose enzyme degradation of bacteria, studied the cellulose enzyme in nature, for cellulose enzyme, which laid the foundation for the development and application.The termites were drawn from Hunan Academy of Forestry Science in this experiment. Cellulase was isolated by pure cultivation, homogenization, centrifugation, sendimentation and column chromatography. The molecular weight, optimum temperature, stability to temperature and pH value and the effect of metal ion on enzyme activity were all analyzed in order to provide theoretical basis to the industrialization of cellulase.MethodThe termites is after the withdrawal of the train by the way, and enriched by the functions of the filter to break down cellulose culture. in order to know the purpose of culture growth and yield enzymes and shake the bottle in the process of bacteria to develop and grow the best suited to the media and trained by the time and are determined to shake the bottle of best service. SDS-PAGE results show that the purification of the enzyme a fiber from the base of the base of the size is about 44.7 kda. Fermentation is cellulose enzymes is, after the sieve chromatography and wait for the purification procedure, the living relative pH4-9 within the scope of the enzyme activity can keep it, the enzymes to hemoglobin purification, polyacrylamide gel electrophoresis for testing the purity and denatured electrophoresis measure for the average, the enzymes the optimum temperature and pH and optimum quality research, the enzymes can improve the response of the environment, the enzymes activity.Result1.By regular filter, the number of able to break down cellulose fungal and degradation of the ability to choose the better Z-165 for the experiment.2.The optimun culture of Z-165 is peptone, pure and the desire and remembering, G-100 sieve chromatography and separation, purification, Foster the conditions for optimum temperature of 40℃, pH 6.0 and oscillating speed and 121 rpm for 24 h. the conditions for the acquisition of the enzyme activity is the average for3647 U/mg.3.Organic is four times the size of the concentration in cellulose of enzyme activity, the higher levels of protein by the deposited sepharose G-100 sieve chromatography and isolated electrophoresis pure enzymes to hemoglobin in multiples. cellulase in temperature 40℃. Low temperature for a living on the 40℃the enzymes can be kept within 20 h have a higher activeity and pH on a significant impact. Metal and organic ion with a response is a great influence to the fibre enzymes, Mg2+ and Cu2+ enzymes response to the curb, and there are obvious Ca2+Zn2+and activation Fe3+, and Na+, K+, there is no significant effect.