Functional Characterization Of The Ated1 ( Embryo Defective 1) Gene Involved In Embryogenesis In Arabidopsis

Embryo development is the basis for the formation of plant growth. Scientists have carried out extensive research on plant embryogenesis genes, some genes associated with early embryonic morphogenesis. In the process of identification of these genes found at least 250 embryos defective gene ( embryo-defective genes, EMBs) essential for the normal development of embryos. These genes were involved in cell growth, metabolism, transport, transcription and translation process.In this study, we isolated a mutant from T-DNA insertion mutant library in Arabidopsis thaliana, which named ated1 (Arabidopsis embryo defective 1) . ed1 embryos stopped developing in the early globular stage, and ultimately abortion. In addition, both embryo and endosperm is abnormal in ed1 mutant. ED1 encode a unknown protein, amino acid sequence analysis showed that it contains C2H2 conserved domain. The gene expression pattern analysis showed that ED1 mainly expressed in young leaves, seed pods, and mature plants petiole, pedicel, stem and other parts of the microstructure; in the ovule it is widely expressed in embryo and endosperm. Subcellular location study showed that ED1 was localized to the nucleus. Finally, we use yeast two-hybrid initial screened 14 proteins, which potential interact with ED1, including ACT12, MYB7, MPK18, ubiquitin 10 and other proteins.These results showed that the ED1 gene function in Arabidopsis embryo and endosperm development, and the mutant cause abnormal embryo abortion, that ED1 gene plays an important role in Arabidopsis embryos during early development.