Preparation And Preservation Of Bilayer Lipid Membrane And The Interaction With Gemini Surfactant

Biomembrane is very important in the whole life procedure. As we would like to know more about life science, the most important is we should know what happened on the Biomembrane. It is very difficult to observe and study it on the original location for the reason that the components and structure and function of Biomembrane is very complicated. So firstly Model membrane system should be made in the laboratory. Bilayer lipid membrane (BLM), as the model system of Biomembrane, not only has the same main structure with biomembrane, but also owns the advantage that is not so complicated, which is an ideal model for Biomembrane Research. Recently, BLM is got more attention by the people in this industry as Biomember Model Research. In environmental pollution test and Life activate substance checking and Interaction between medication and biomembrane, BLM has very good research future. But until now, the membrane stability performance of BLM is not so good and which can not be used in Science Experiment and Industry Application etc. In order to solve this problem, this article offered some solution for improving BLM stability performance and longer life cycle. In the meantime for the reason that Biochemical Reaction on the membrane with the base of BLM is very important in the research, this article has synthesize new Gemini Surfactant, BLM by metal supported as the base, which studied the interaction between Gemini Surfactant and BLM by Electrochemistry analyzer in order to get related information for the studying the interaction between Medication and BLM.The main result from the article:(1) Take Tetradecyl trimethyl ammonium bromide and N,N,N',N'-Tetramethyl-ethylenediamine as raw material, one kind of new Gemini Surfactant [C₁₄-2-C₁₄·2Br^-] was compounded; Acetoacetate / Alcohol was used to purified the Surfactant. The study by Fourier Transform Infrared Spectrometry and Nuclear Magnetic Resonance Spectroscopy indicated that the Gemini Surfactant[C₁₄-2-C₁₄· 2Br^-] has very high purification.(2) Stable Bilayer lipid membrane is created on the cross section of stainless steel by making use of Lecithin's n-Decane solution as the membrane forming liquid, moreover if cholesterin is added into the forming liquid, the membrane forming efficiency can be improved and the membrane will be more stable. The Bilayer Lipid Membrane Forming area also can be expanded by taking abrasive cloth to polish the stainless steel cross section.(3) With the way of freezing the Bilayer Lipid Membrane stage by stage, the preservation period of Bilayer Lipid Membrane can be more longer, and the vibration affection to Bilayer Lipid Membrane from environment can be decreased consumedly (4)Tetradecyl trimethyl ammonium bromide and Gemini surfactant C₁₄-2-C₁₄·2Br^-, as Surfactant, both of them can change the structure of Bilayer Lipid Membrane. When the concentration is in the range of 0.0004%-0.015%, Tetradecyl trimethyl ammonium bromide will decrease the membrane resistance and increase the membrane capacitance, finally, membrane resistance and capacitance will go to a fixed value; when the concentration is in the range of 0.0004%-0.015%, Gemini surfactant C₁₄-2-C₁₄·2Br^- has the similar affection to Lecithin Bilayer Lipid Membrane as Tetradecyl trimethyl ammonium bromide. But to the Bilayer Lipid Membrane integrated with cholesterol, this dose not lower the resistance but increase it, there is no good rule that can be found in the membrane capacitance change. Finally membrane resistance and capacitance all will go to a fixed value.