| The fabrication of ultr-thin film plays a critial role in the appliocation of functional compounds.Traditionally, Langmuir-Blodgett film, Self-assembly film, vacuum evaporation film, and the biolayers lipid film are the mainly method of the preparation of ultra-thin films. Self-assembled ultra-thin film attaracts great interest because their structures are simple and clear, and the preparation methods are usually easy to be approached. As an prototype of certain ultrathin film apparatus, LB film is closely related to chemistry, physics, biology, medicinal, electrodynamic, and optical researches in forming the intersection of those subjects.Either from new materials, biotechnology, microelectronics, or from the point of view, LB film is regarded as an ideal ultrathin films since the molecules in LB films also has highly ordered arrangement. As the carrier of genetic information, DNAs are also regard as a natural nano-scale molecule duce to their unique morphology properties and specific molecular recognition ability. DNA-based nano-structure has grasped novel attention now a day. In this subject, The self-assembled DNA Langmuir-Blodgett(LB) patterns were deposited on solid substrate through LB Langmuir-Blodgett technique. We studied the conformational changes and the phase transition of DNA LB films under different pressures. The shape of DNA molecules under different forming pressure were characterized by atomic force microscopy technique. Experimental results suggest that the DNA molecule has the characteristics of self-assembly. A molecular fluorescence spectroscopy of DNA molecule LB film was obtained in our experiments. we fund that fluorescence intensity was enhanced from the molecular fluorescence spectroscopy of DNA molecule LB film. Enhancement of fluorescence is possible with the ordered structure of DNA LB films. We introduced another DNA molecules-Lambda DNA to self-assembly at the air/water interface, and obtained more ordered DNA molecules template. |
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